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Literature summary extracted from

  • Schnee, C.; Köllner, T.G.; Gershenzon, J.; Degenhardt, J.
    The maize gene terpene synthase 1 encodes a sesquiterpene synthase catalyzing the formation of (E)-beta-farnesene, (E)-nerolidol, and (E,E)-farnesol after herbivore damage (2002), Plant Physiol., 130, 2049-2060.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.49 overexpression in Escherichia coli Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.49 0.001
-
 (2E,6E)-farnesyl diphosphate 30°C Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.49 Mg2+ divalent metal ion cofactor is required for enzyme activity, KM-value for Mg2+: 0.47 mM. Mg2+ and Mn2+ give substantial activity, with Mg2+ at 5 mM giving the highest activity Zea mays
4.2.3.49 Mg2+ divalent metal ion cofactor is required for enzyme activity, KM-value for Mn2+: 0.026 mM. Mg2+ and Mn2+ give substantial activity, with Mg2+ at 5 mM giving the highest activity Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.49 67400
-
 1 * 67400, calculated from sequence Zea mays
4.2.3.49 67400
-
 2 * 67400, calculated from sequence Zea mays
4.2.3.49 71000
-
 monomer, gel filtration Zea mays
4.2.3.49 140000
-
 dimer, recombinant enzyme, gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.49 (2E,6E)-farnesyl diphosphate + H2O Zea mays herbivore-induced production of (3R,6E)-nerolidol, an intermediate in the formation of 4,8-dimethyl-1,3(E),7-nonatriene (3R,6E)-nerolidol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.49 Zea mays Q84ZW8 cultivar B73 and Delprim
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.49 leaf transcripts are detected in the leaf and sheath tissue of 2-week-old uninjured maize plants, but none is found in the roots. After 16 h of herbivory by Egyptian cotton leafworm larvae, transcript levels in the leaves of maize cv B73 are induced about 8-fold. The tps1 transcript levels in the maize cv Delprim are high even in uninjured plants and are only marginally elevated by herbivory. In older plants (3 months), moderate levels of transcript are present in leaves of both herbivore-treated and untreated plants of both cultivars. 16 hours after the initiation of herbivore damage, there is a significant elevation in transcript level Zea mays
-

Storage Stability

EC Number Storage Stability Organism
4.2.3.49 -80°C, stable for at least 1 week Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.49 (2E,6E)-farnesyl diphosphate + H2O herbivore-induced production of (3R,6E)-nerolidol, an intermediate in the formation of 4,8-dimethyl-1,3(E),7-nonatriene Zea mays (3R,6E)-nerolidol + diphosphate
-
 ?
4.2.3.49 (2E,6E)-farnesyl diphosphate + H2O in the presence of geranyl diphosphate the enzyme catalyzes the formation of (3R)-linalool and geraniol Zea mays (3R,6E)-nerolidol + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.49 dimer 2 * 67400, calculated from sequence Zea mays
4.2.3.49 monomer 1 * 67400, calculated from sequence Zea mays

Synonyms

EC Number Synonyms Comment Organism
4.2.3.49 terpene synthase 1
-
 Zea mays
4.2.3.49 TPS1
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.49 30 37
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.49 7
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.49 6 7.7 half-maximal activity at pH 6.0 and pH 7.7 Zea mays

Expression

EC Number Organism Comment Expression
4.2.3.49 Zea mays transcripts are detected in the leaf and sheath tissue of 2-week-old uninjured maize plants, but none is found in the roots. After 16 h of herbivory by Egyptian cotton leafworm larvae, transcript levels in the leaves of maize cv B73 are induced about 8-fold. The tps1 transcript levels in the maize cv Delprim are high even in uninjured plants and are only marginally elevated by herbivory. In older plants (3 months), moderate levels of transcript are present in leaves of both herbivore-treated and untreated plants of both cultivars up