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Literature summary extracted from

  • Maeda, H.; Shasany, A.K.; Schnepp, J.; Orlova, I.; Taguchi, G.; Cooper, B.R.; Rhodes, D.; Pichersky, E.; Dudareva, N.
    RNAi suppression of arogenate dehydratase1 reveals that phenylalanine is synthesized predominantly via the arogenate pathway in petunia petals (2010), Plant Cell, 22, 832-849.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.91 coding regions corresponding to mature ADT protein (lacking the N-terminal plastid transit peptides) subcloned into the expression vector pET-28a, which contains an N-terminal 6xHis tag. Recombinant protein expressed in Escherichia coli Rosetta cells Petunia x hybrida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.91 0.0488
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 0.0667
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 0.179
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 0.4658
-
 prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 0.752
-
 prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.91 chloroplast
-
 Petunia x hybrida 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.91 43400
-
 sequence analysis Petunia x hybrida
4.2.1.91 45900
-
 sequence analysis Petunia x hybrida
4.2.1.91 47300
-
 sequence analysis Petunia x hybrida

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.91 Petunia x hybrida D3U715
-
-
4.2.1.91 Petunia x hybrida D3U716
-
-
4.2.1.91 Petunia x hybrida D3U717
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.91 by fast protein liquid chromatography and gel filtration Petunia x hybrida

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.91 flower
-
 Petunia x hybrida
-
4.2.1.91 leaf
-
 Petunia x hybrida
-
4.2.1.91 petal
-
 Petunia x hybrida
-
4.2.1.91 stem only ADT1 and ADT3 Petunia x hybrida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.91 L-arogenate
-
 Petunia x hybrida L-phenylalanine + CO2 + H2O
-
 ?
4.2.1.91 L-arogenate ADT1 shows strict substrate specificity toward arogenate, although with the lowest catalytic efficiency among the three ADTs Petunia x hybrida L-phenylalanine + CO2 + H2O
-
 ?
4.2.1.91 prephenate
-
 Petunia x hybrida phenylpyruvate + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.91 ADT1
-
 Petunia x hybrida
4.2.1.91 ADT2
-
 Petunia x hybrida
4.2.1.91 ADT3
-
 Petunia x hybrida
4.2.1.91 arogenate dehydratase1
-
 Petunia x hybrida
4.2.1.91 arogenate dehydratase2
-
 Petunia x hybrida
4.2.1.91 arogenate dehydratase3
-
 Petunia x hybrida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.91 0.012
-
 prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 0.06
-
 prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 0.194
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 0.267
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 1.231
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida

Expression

EC Number Organism Comment Expression
4.2.1.91 Petunia x hybrida ADT1 suppression via RNA interference in petunia petals significantly reduces ADT activity, levels of Phe, and downstream phenylpropanoid/benzenoid volatiles, whereby arogenate levels are unaltered, while shikimate and Trp levels are decreased in transgenic petals down
4.2.1.91 Petunia x hybrida ADT1 exhibits the highest level of expression in corolla and tube, the scentproducing parts of petunia flowers. In corolla, the ADT1 transcript levels exceed those of ADT2 and ADT3 by 88- and 10fold, respectively. ADT1 expression is positively correlated with endogenous Phe levels throughout flower development up
4.2.1.91 Petunia x hybrida in corolla, the ADT1 transcript levels exceed those of ADT2 and ADT3 by 88- and 10fold, respectively up

General Information

EC Number General Information Comment Organism
4.2.1.91 malfunction ADT1 suppression leads to downregulation of carbon flux toward shikimic acid, but exogenous supply of shikimate bypasses this negative regulation and results in elevated arogenate accumulation Petunia x hybrida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.91 0.03
-
 prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 0.08
-
 prephenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 1.48
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 4
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida
4.2.1.91 18.51
-
 L-arogenate at 37°C, in 250 mM Na-phosphate buffer, pH 8.2, 1 mM EDTA Petunia x hybrida