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Literature summary extracted from

  • Brash, A.
    Mechanistic aspects of CYP74 allene oxide synthases and related cytochrome P450 enzymes (2009), Phytochemistry, 70, 1522-1531.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.92 additional information exhibits weak activity prior to activation (in vitro), can be massively activated with detergent Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.92
-
 Parthenium argentatum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.92
-
 Arabidopsis thaliana
4.2.1.92 is a typical P450, the loop forms a compact beta-turn and incorporates the most typical P450 consensus sequence, FXXG, followed by three residues (XRX) followed by CXG encompassing the cysteine. CYP74 heme is strongly hold in place than usual via ionic interactions with its proprionate groups Parthenium argentatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.92 heme CYP74 heme is strongly hold in place than usual via ionic interactions with its proprionate groups Parthenium argentatum
4.2.1.92 Iron
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.92 53000
-
-
 Parthenium argentatum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.92 Acaryochloris marina
-
-
-
4.2.1.92 Arabidopsis thaliana
-
-
-
4.2.1.92 Linum usitatissimum
-
 seed
-
4.2.1.92 Parthenium argentatum
-
-
-
4.2.1.92 Solanum lycopersicum
-
-
-
4.2.1.92 Solanum tuberosum
-
-
-
4.2.1.92 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.92
-
 Linum usitatissimum
4.2.1.92
-
 Parthenium argentatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.92 additional information CYP74A2 is most abundant in rubber, accounting for about 50% of the protein content Parthenium argentatum
-
4.2.1.92 seed
-
 Linum usitatissimum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.92 additional information CYP74 enzymes use their fatty acid hydroperoxide substrate to activate the enzyme and substrate in one and the same step Linum usitatissimum ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.92 allene oxide synthase
-
 Zea mays
4.2.1.92 allene oxide synthase
-
 Solanum tuberosum
4.2.1.92 allene oxide synthase
-
 Arabidopsis thaliana
4.2.1.92 allene oxide synthase
-
 Solanum lycopersicum
4.2.1.92 allene oxide synthase
-
 Linum usitatissimum
4.2.1.92 allene oxide synthase
-
 Parthenium argentatum
4.2.1.92 allene oxide synthase
-
 Acaryochloris marina
4.2.1.92 AOS
-
 Zea mays
4.2.1.92 AOS
-
 Solanum tuberosum
4.2.1.92 AOS
-
 Arabidopsis thaliana
4.2.1.92 AOS
-
 Solanum lycopersicum
4.2.1.92 AOS
-
 Linum usitatissimum
4.2.1.92 AOS
-
 Parthenium argentatum
4.2.1.92 AOS
-
 Acaryochloris marina
4.2.1.92 CYP74
-
 Linum usitatissimum
4.2.1.92 CYP741
-
 Arabidopsis thaliana
4.2.1.92 CYP742
-
 Parthenium argentatum
4.2.1.92 CYP74A
-
 Zea mays
4.2.1.92 CYP74c
-
 Solanum tuberosum
4.2.1.92 CYP74c
-
 Solanum lycopersicum

General Information

EC Number General Information Comment Organism
4.2.1.92 metabolism role for Phe137 in catalysis, mutation to Leu switches the product outcome from AOS to hydroperoxide lyase Arabidopsis thaliana
4.2.1.92 physiological function is a cytochrome P450 Linum usitatissimum
4.2.1.92 physiological function is a potential rubber transferase, the enzyme polymerizes thousands of isoprenes into molecules of rubber. Plays no role in jasmonate biosynthesis Parthenium argentatum
4.2.1.92 physiological function plays a role in jasmonate biosynthesis Zea mays
4.2.1.92 physiological function plays a role in jasmonate biosynthesis Solanum tuberosum
4.2.1.92 physiological function plays a role in jasmonate biosynthesis Solanum lycopersicum