BRENDA - Enzyme Database show

Alteration of the substrates prefolded conformation and cyclization stereochemistry of oxidosqualene-lanosterol cyclase of Saccharomyces cerevisiae by substitution at phenylalanine 699

Wu, T.K.; Chang, C.H.; Wen, H.Y.; Liu, Y.T.; Li, W.H.; Wang, T.T.; Shie, W.S.; Org. Lett. 12, 500-503 (2010)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.99.7
F699H
the mutant produces lanosterol (13%), protosta-13(17)-dien-3beta-ol (70%), and (17Z)-protosta-17(20),24-dien-3beta-ol (17%)
Saccharomyces cerevisiae
5.4.99.7
F699I
the mutant produces lanosterol (100%) as the wild type enzyme
Saccharomyces cerevisiae
5.4.99.7
F699L
the mutant produces lanosterol (100%) as the wild type enzyme
Saccharomyces cerevisiae
5.4.99.7
F699M
the mutant produces lanosterol (13%), (17Z)-protosta-17(20),24-dien-3beta-ol (46%), protosta-13(17)-dien-3beta-ol (70%), malabarica-14E,17E,21-trien-3beta-ol (7%), 17alpha-protosta-20,24-dien-3beta-ol (1%), (17Z)-protosta-17(20),24-dien-3beta-ol (10%), (13alphaH)-isomalabarica-14E,17E,21-dien-3 beta-ol (17%), and (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol (18%)
Saccharomyces cerevisiae
5.4.99.7
F699N
nonviable mutant, does not produce lanosterol, but protosta-13(17)-dien-3beta-ol (55%), malabarica-14E,17E,21-trien-3beta-ol (5%), 17alpha-protosta-20,24-dien-3beta-ol (24%), and (17Z)-protosta-17(20),24-dien-3beta-ol (16%)
Saccharomyces cerevisiae
5.4.99.7
F699P
the mutant produces lanosterol (100%) as the wild type enzyme
Saccharomyces cerevisiae
5.4.99.7
F699T
the mutant produces lanosterol (less than 0.2%) and protosta-13(17)-dien-3beta-ol (above 99.8%)
Saccharomyces cerevisiae
5.4.99.7
F699X
TKW14[pERG7F699X] site-saturated mutants allow for ergosterol-independent growth, with the exception of Leu, Ile, His, Met, Pro, and Thr substitutions
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.7
Saccharomyces cerevisiae
-
-
-
5.4.99.7
Saccharomyces cerevisiae TKW14
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.7
(3S)-2,3-oxidosqualene
-
706053
Saccharomyces cerevisiae
lanosterol
-
-
-
?
5.4.99.7
(3S)-2,3-oxidosqualene
-
706053
Saccharomyces cerevisiae TKW14
lanosterol
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.99.7
F699H
the mutant produces lanosterol (13%), protosta-13(17)-dien-3beta-ol (70%), and (17Z)-protosta-17(20),24-dien-3beta-ol (17%)
Saccharomyces cerevisiae
5.4.99.7
F699I
the mutant produces lanosterol (100%) as the wild type enzyme
Saccharomyces cerevisiae
5.4.99.7
F699L
the mutant produces lanosterol (100%) as the wild type enzyme
Saccharomyces cerevisiae
5.4.99.7
F699M
the mutant produces lanosterol (13%), (17Z)-protosta-17(20),24-dien-3beta-ol (46%), protosta-13(17)-dien-3beta-ol (70%), malabarica-14E,17E,21-trien-3beta-ol (7%), 17alpha-protosta-20,24-dien-3beta-ol (1%), (17Z)-protosta-17(20),24-dien-3beta-ol (10%), (13alphaH)-isomalabarica-14E,17E,21-dien-3 beta-ol (17%), and (13alphaH)-isomalabarica-14Z,17E,21-dien-3beta-ol (18%)
Saccharomyces cerevisiae
5.4.99.7
F699N
nonviable mutant, does not produce lanosterol, but protosta-13(17)-dien-3beta-ol (55%), malabarica-14E,17E,21-trien-3beta-ol (5%), 17alpha-protosta-20,24-dien-3beta-ol (24%), and (17Z)-protosta-17(20),24-dien-3beta-ol (16%)
Saccharomyces cerevisiae
5.4.99.7
F699P
the mutant produces lanosterol (100%) as the wild type enzyme
Saccharomyces cerevisiae
5.4.99.7
F699T
the mutant produces lanosterol (less than 0.2%) and protosta-13(17)-dien-3beta-ol (above 99.8%)
Saccharomyces cerevisiae
5.4.99.7
F699X
TKW14[pERG7F699X] site-saturated mutants allow for ergosterol-independent growth, with the exception of Leu, Ile, His, Met, Pro, and Thr substitutions
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.7
(3S)-2,3-oxidosqualene
-
706053
Saccharomyces cerevisiae
lanosterol
-
-
-
?
5.4.99.7
(3S)-2,3-oxidosqualene
-
706053
Saccharomyces cerevisiae TKW14
lanosterol
-
-
-
?