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Literature summary extracted from

  • Iraha, F.; Oki, K.; Kobayashi, T.; Ohno, S.; Yokogawa, T.; Nishikawa, K.; Yokoyama, S.; Sakamoto, K.
    Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion (2010), Nucleic Acids Res., 38, 3682-3691.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.1.1.1 biotechnology the present engineering allows Escherichia coli TyrRS variants for non-natural amino acids to be developed in Escherichia coli, for use in both eukaryotic and bacterial cells for genetic code expansion Saccharomyces cerevisiae
6.1.1.1 biotechnology the present engineering allows Escherichia coli TyrRS variants for non-natural amino acids to be developed in Escherichia coli, for use in both eukaryotic and bacterial cells for genetic code expansion Methanocaldococcus jannaschii
6.1.1.1 biotechnology the present engineering allows Escherichia coli TyrRS variants for non-natural amino acids to be developed in Escherichia coli, for use in both eukaryotic and bacterial cells for genetic code expansion Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.1 into the vectors pACYC184 and pAp102 Saccharomyces cerevisiae
6.1.1.1 into the vectors pACYC184 and pAp102 Methanocaldococcus jannaschii
6.1.1.1 into the vectors pACYC184 and pAp102 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.1 the iodoTyrRS-ec-3-azide-L-tyrosine structure is determined at a resolution of 1.8 A Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.1 additional information the Escherichia coli TyrRS-tRNATyr pair is functionally replaced by the Methanocaldococcus jannaschii tyrosine pair Methanocaldococcus jannaschii
6.1.1.1 additional information the Escherichia coli TyrRS-tRNATyr pair is functionally replaced by the Saccharomyces cerevisiae tyrosine pair Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr Saccharomyces cerevisiae
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr Escherichia coli
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.1 Escherichia coli P0AGJ9
-
-
6.1.1.1 Methanocaldococcus jannaschii
-
-
-
6.1.1.1 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.1
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.1 ATP + 3-azido-L-tyrosine + tRNATyr
-
 Saccharomyces cerevisiae AMP + diphosphate + 3-azido-L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + 3-azido-L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + 3-azido-L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + 3-azido-L-tyrosine + tRNATyr
-
 Escherichia coli AMP + diphosphate + 3-azido-L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + 3-iodo-L-tyrosine + tRNATyr
-
 Saccharomyces cerevisiae AMP + diphosphate + 3-iodo-L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + 3-iodo-L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + 3-iodo-L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + 3-iodo-L-tyrosine + tRNATyr
-
 Escherichia coli AMP + diphosphate + 3-iodo-L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Saccharomyces cerevisiae AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Escherichia coli AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.1 Tyrosyl-tRNA synthetase
-
 Saccharomyces cerevisiae
6.1.1.1 Tyrosyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.1 Tyrosyl-tRNA synthetase
-
 Escherichia coli
6.1.1.1 TyrRS
-
 Saccharomyces cerevisiae
6.1.1.1 TyrRS
-
 Methanocaldococcus jannaschii
6.1.1.1 TyrRS
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.1 ATP
-
 Saccharomyces cerevisiae
6.1.1.1 ATP
-
 Methanocaldococcus jannaschii
6.1.1.1 ATP
-
 Escherichia coli