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Literature summary extracted from

  • Chu, H.M.; Ko, T.P.; Wang, A.H.
    Crystal structure and substrate specificity of plant adenylate isopentenyltransferase from Humulus lupulus: distinctive binding affinity for purine and pyrimidine nucleotides (2010), Nucleic Acids Res., 38, 1738-1748.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.27 overexpressed as a N-terminal hexahistidine tag protein in Escherichia coli BL21 (DE3) Humulus lupulus
2.5.1.75 overexpressed as a N-terminal hexahistidine tag protein in Escherichia coli BL21 (DE3) Humulus lupulus
2.5.1.112 expressed in Escherichia coli BL21(DE3) cells Humulus lupulus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.27 The crystal structure of the AIPT-ATP complex from Humulus lupulus is similar to the previous structures of Agrobacterium AIPT and yeast tRNA-IPT. The enzyme is structurally homologous to the NTP-binding kinase family of proteins but forms a solvent-accessible channel that binds to the donor substrate dimethylallyl diphosphate, which is directed toward the acceptor substrate ATP/ADP. Humulus lupulus
2.5.1.75 The crystal structure of the AIPT-ATP complex from Humulus lupulus is similar to the previous structures of Agrobacterium AIPT and yeast tRNA-IPT. The enzyme is structurally homologous to the NTP-binding kinase family of proteins but forms a solvent-accessible channel that binds to the donor substrate dimethylallyl diphosphate, which is directed toward the acceptor substrate ATP/ADP. Humulus lupulus
2.5.1.112 in complex with ATP, sitting drop vapor diffusion method, using 0.2 M ammonium tartrate and 20% (w/v) PEG 3350 Humulus lupulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.112 0.0162
-
 ATP in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C Humulus lupulus
2.5.1.112 0.0193
-
 ADP in HEPES (20mM, pH 7.0; 150 mM NaCl, 3 mM dithiothreitol), at 25°C Humulus lupulus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + AMP Humulus lupulus
-
 diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
 ir
2.5.1.27 dimethylallyl diphosphate + ATP Humulus lupulus
-
 N6-(dimethylallyl) adenosine 5'-triphosphate + diphosphate
-
 ir
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA Humulus lupulus
-
 diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ir
2.5.1.112 dimethylallyl diphosphate + ADP Humulus lupulus
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP Humulus lupulus the enzme favors ATP as its ligand diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Humulus lupulus Q5GHF7
-
-
2.5.1.75 Humulus lupulus Q5GHF7
-
-
2.5.1.112 Humulus lupulus Q5GHF7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.112 Ni-NTA resin column chromatography and Mono Q column chromatography Humulus lupulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + AMP
-
 Humulus lupulus diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
 ir
2.5.1.27 dimethylallyl diphosphate + ATP
-
 Humulus lupulus N6-(dimethylallyl) adenosine 5'-triphosphate + diphosphate
-
 ir
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA
-
 Humulus lupulus diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ir
2.5.1.112 dimethylallyl diphosphate + ADP
-
 Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP the enzme favors ATP as its ligand Humulus lupulus diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + CTP
-
 Humulus lupulus ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + dATP the enzyme shows preference for dATP over ADP and 80% activity with dATP compared to ATP Humulus lupulus ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + GTP
-
 Humulus lupulus ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + UTP
-
 Humulus lupulus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.27 adenylate isopentenyltransferase
-
 Humulus lupulus
2.5.1.27 AIPT
-
 Humulus lupulus
2.5.1.27 HlAIPT adenylate isopentenyltransferase-ATP complex from Humulus lupulus Humulus lupulus
2.5.1.112 adenylate isopentenyltransferase
-
 Humulus lupulus
2.5.1.112 AIPT
-
 Humulus lupulus

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.112 ADP
-
 Humulus lupulus
2.5.1.112 ATP
-
 Humulus lupulus

General Information

EC Number General Information Comment Organism
2.5.1.27 metabolism initial reaction in the biosynthesis of cytokinins, which are important plant hormones Humulus lupulus