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Literature summary extracted from

  • Ikeuchi, Y.; Kimura, S.; Numata, T.; Nakamura, D.; Yokogawa, T.; Ogata, T.; Wada, T.; Suzuki, T.; Suzuki, T.
    Agmatine-conjugated cytidine in a tRNA anticodon is essential for AUA decoding in archaea (2010), Nat. Chem. Biol., 6, 277-282.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.22 expression in Escherichia coli Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.22 C352A/C355A almost no 2-agmatinylcytidine formation is observed Archaeoglobus fulgidus
6.3.4.22 G218A mutation reduces activity Archaeoglobus fulgidus
6.3.4.22 R140/G141A mutation completely abolishes activity Archaeoglobus fulgidus
6.3.4.22 R164A mutation reduces activity Archaeoglobus fulgidus
6.3.4.22 R217A mutation reduces activity Archaeoglobus fulgidus
6.3.4.22 T248A/D249A mutation reduces activity Archaeoglobus fulgidus
6.3.4.22 Y163A mutation reduces activity Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.22 ATP + agmatine + [tRNAIle2]-cytidine34 Archaeoglobus fulgidus
-
[tRNAIle2]-2-agmatinylcytidine34 + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.22 Archaeoglobus fulgidus O28025
-
-
6.3.4.22 Pyrococcus horikoshii O59476
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.22
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.22 ATP + agmatine + [tRNAIle2]-cytidine34
-
Archaeoglobus fulgidus [tRNAIle2]-2-agmatinylcytidine34 + ?
-
?
6.3.4.22 ATP + agmatine + [tRNAIle2]-cytidine34
-
Pyrococcus horikoshii [tRNAIle2]-2-agmatinylcytidine34 + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.22 AF2259
-
Archaeoglobus fulgidus
6.3.4.22 AF2259
-
Pyrococcus horikoshii
6.3.4.22 TiaS
-
Archaeoglobus fulgidus
6.3.4.22 TiaS
-
Pyrococcus horikoshii
6.3.4.22 tRNAIle-2-agmatinylcytidine synthetase
-
Archaeoglobus fulgidus
6.3.4.22 tRNAIle-2-agmatinylcytidine synthetase
-
Pyrococcus horikoshii
6.3.4.22 tRNAIle-agm2C synthetase
-
Archaeoglobus fulgidus
6.3.4.22 tRNAIle-agm2C synthetase
-
Pyrococcus horikoshii
6.3.4.22 tRNAIle-agmatidine synthetase
-
Archaeoglobus fulgidus
6.3.4.22 tRNAIle-agmatidine synthetase
-
Pyrococcus horikoshii

General Information

EC Number General Information Comment Organism
6.3.4.22 physiological function modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine Archaeoglobus fulgidus
6.3.4.22 physiological function modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine Pyrococcus horikoshii