EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.4.22 | expression in Escherichia coli | Archaeoglobus fulgidus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.3.4.22 | C352A/C355A | almost no 2-agmatinylcytidine formation is observed | Archaeoglobus fulgidus |
6.3.4.22 | G218A | mutation reduces activity | Archaeoglobus fulgidus |
6.3.4.22 | R140/G141A | mutation completely abolishes activity | Archaeoglobus fulgidus |
6.3.4.22 | R164A | mutation reduces activity | Archaeoglobus fulgidus |
6.3.4.22 | R217A | mutation reduces activity | Archaeoglobus fulgidus |
6.3.4.22 | T248A/D249A | mutation reduces activity | Archaeoglobus fulgidus |
6.3.4.22 | Y163A | mutation reduces activity | Archaeoglobus fulgidus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.22 | ATP + agmatine + [tRNAIle2]-cytidine34 | Archaeoglobus fulgidus | - |
[tRNAIle2]-2-agmatinylcytidine34 + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.4.22 | Archaeoglobus fulgidus | O28025 | - |
- |
6.3.4.22 | Pyrococcus horikoshii | O59476 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.4.22 | - |
Archaeoglobus fulgidus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.4.22 | ATP + agmatine + [tRNAIle2]-cytidine34 | - |
Archaeoglobus fulgidus | [tRNAIle2]-2-agmatinylcytidine34 + ? | - |
? | |
6.3.4.22 | ATP + agmatine + [tRNAIle2]-cytidine34 | - |
Pyrococcus horikoshii | [tRNAIle2]-2-agmatinylcytidine34 + ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.4.22 | AF2259 | - |
Archaeoglobus fulgidus |
6.3.4.22 | AF2259 | - |
Pyrococcus horikoshii |
6.3.4.22 | TiaS | - |
Archaeoglobus fulgidus |
6.3.4.22 | TiaS | - |
Pyrococcus horikoshii |
6.3.4.22 | tRNAIle-2-agmatinylcytidine synthetase | - |
Archaeoglobus fulgidus |
6.3.4.22 | tRNAIle-2-agmatinylcytidine synthetase | - |
Pyrococcus horikoshii |
6.3.4.22 | tRNAIle-agm2C synthetase | - |
Archaeoglobus fulgidus |
6.3.4.22 | tRNAIle-agm2C synthetase | - |
Pyrococcus horikoshii |
6.3.4.22 | tRNAIle-agmatidine synthetase | - |
Archaeoglobus fulgidus |
6.3.4.22 | tRNAIle-agmatidine synthetase | - |
Pyrococcus horikoshii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
6.3.4.22 | physiological function | modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine | Archaeoglobus fulgidus |
6.3.4.22 | physiological function | modification at the anticodon wobble position of archaeal tRNAIle2. According to the chemical structure of agm2C, conjugation of an agmatine moiety at the C2 position of cytosine induces a tautomeric conversion with protonation of the N3 position and imino group formation at the C4 position. This modification completely alters the proton donor-acceptor pattern of cytosine in Watson-Click hydrogen bonding to that of uracil, enabling agm2C to base pair with adenine instead of guanine. tRNAIle2 acquires the ability to decode the AUA codon via formation 2-agmatinylcytidine | Pyrococcus horikoshii |