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Literature summary extracted from

  • Kim, M.S.; Cho, S.M.; Kang, E.Y.; Im, Y.J.; Hwangbo, H.; Kim, Y.C.; Ryu, C.M.; Yang, K.Y.; Chung, G.C.; Cho, B.H.
    Galactinol is a signaling component of the induced systemic resistance caused by Pseudomonas chlororaphis O6 root colonization (2008), Mol. Plant Microbe Interact., 21, 1643-1653.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.123 agriculture transgenic plants show constitutive resistance against fungal and bacterial pathogens and an increased tolerance to drought and high salinity stresses Cucumis sativus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.123 enzyme DNA as cloned into the predigested binary vector, tobacco is transformed by the leaf-disk method using Agrobacterium tumefaciens LBA4404 containing the CsGolS1 recombinant plasmid Cucumis sativus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.123 UDP-D-galactose + myo-inositol Cucumis sativus
-
 UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.123 Cucumis sativus Q84V66
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.123 UDP-D-galactose + myo-inositol
-
 Cucumis sativus UDP + alpha-D-galactosyl-(1-3)-1D-myo-inositol i.e. galactinol ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.123 CsGolS1
-
 Cucumis sativus
2.4.1.123 galactinol synthase
-
 Cucumis sativus

Expression

EC Number Organism Comment Expression
2.4.1.123 Cucumis sativus overexpression of the CsGolS1 gene confers resistance in transgenic tobacco plants against fungal and bacterial pathogens, the CsGolS1-overexpressing transgenic plants demonstrate constitutive resistance against the pathogens Botrytis cinerea and Erwinia carotovora, and they show an increased accumulation in galactinol content, the CsGolS1-overexpressing transgenic plants demonstrate an increased tolerance to drought and high salinity stresses additional information
2.4.1.123 Cucumis sativus at 3 to 5 days after inoculation with Botrytis cinerea, the CsGolS1 overexpressors show more resistance to the pathogen infection (40 to 63% increase in the survival rate) compared with wild-type plants, expression of a galactinol synthase gene is primed in the leaves of cucumber plants by Pseudomonaschlororaphis O6 root colonization, the transcript of the Cucumis sativus induced systemic resistance gene 3 (CsISR3) clone, encoding a cucumber galactinol synthase is accumulated after a challenge inoculation with Corynespora cassiicola up