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Literature summary extracted from

  • Sureka, K.; Sanyal, S.; Basu, J.; Kundu, M.
    Polyphosphate kinase 2: a modulator of nucleoside diphosphate kinase activity in mycobacteria (2009), Mol. Microbiol., 74, 1187-1197.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.1 expressed in Escherichia coli BL21(DE3) cells Mycolicibacterium smegmatis
2.7.4.1 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 F67A 84% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 G69R 67.1% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 G74R 7.6% activity compared to the wild type enzyme, the mutant enzyme cannot interact with nucleoside diphosphate kinase Mycobacterium tuberculosis
2.7.4.1 H115A 49% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 H115A/H247A 6% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 H247A 38% activity compared to the wild type enzyme Mycobacterium tuberculosis
2.7.4.1 K75A 89% activity compared to the wild type enzyme Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.1 membrane
-
 Mycolicibacterium smegmatis 16020
-
2.7.4.1 membrane
-
 Mycobacterium tuberculosis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.1 additional information Mycolicibacterium smegmatis PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase ?
-
 ?
2.7.4.1 additional information Mycobacterium tuberculosis PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase ?
-
 ?
2.7.4.1 additional information Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase ?
-
 ?
2.7.4.1 additional information Mycobacterium tuberculosis H37Rv PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Mycobacterium tuberculosis
-
-
-
2.7.4.1 Mycobacterium tuberculosis H37Rv
-
-
-
2.7.4.1 Mycolicibacterium smegmatis
-
-
-
2.7.4.1 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.1 Ni2+-NTA agarose column chromatography Mycolicibacterium smegmatis
2.7.4.1 Ni2+-NTA agarose column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 GDP + (phosphate)n+1 PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro Mycolicibacterium smegmatis GTP + (phosphate)n
-
 ?
2.7.4.1 GDP + (phosphate)n+1 PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro Mycobacterium tuberculosis GTP + (phosphate)n
-
 ?
2.7.4.1 GDP + (phosphate)n+1 PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 GTP + (phosphate)n
-
 ?
2.7.4.1 GDP + (phosphate)n+1 PPK2 catalyses the synthesis of GTP from GDP using polyphosphate rather than ATP as phosphate donor, PPK2 preferentially synthesizes GTP over CTP or UTP in vitro Mycobacterium tuberculosis H37Rv GTP + (phosphate)n
-
 ?
2.7.4.1 additional information PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase Mycolicibacterium smegmatis ?
-
 ?
2.7.4.1 additional information PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase Mycobacterium tuberculosis ?
-
 ?
2.7.4.1 additional information PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?
2.7.4.1 additional information PPK2 interacts with and modulates nucleotide-synthesizing activity of nucleoside diphosphate kinase Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 polyphosphate kinase 2
-
 Mycolicibacterium smegmatis
2.7.4.1 polyphosphate kinase 2
-
 Mycobacterium tuberculosis
2.7.4.1 PPK2
-
 Mycolicibacterium smegmatis
2.7.4.1 PPK2
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.4.1 GDP
-
 Mycolicibacterium smegmatis
2.7.4.1 GDP
-
 Mycobacterium tuberculosis