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Literature summary extracted from

  • Blake, K.L.; ONeill, A.J.; Mengin-Lecreulx, D.; Henderson, P.J.; Bostock, J.M.; Dunsmore, C.J.; Simmons, K.J.; Fishwick, C.W.; Leeds, J.A.; Chopra, I.
    The nature of Staphylococcus aureus MurA and MurZ and approaches for detection of peptidoglycan biosynthesis inhibitors (2009), Mol. Microbiol., 72, 335-343.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.7 drug development cell-wall synthesis is an important target for antibacterial agents, and MurA is one of the few enzymes involved in the cytoplasmic stage of peptidoglycan biosynthesis which is specifically inhibited by an existing antimicrobial agent Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 the murA gene is cloned and overexpressed in Escherichia coli with a C-terminal 6-His tag Staphylococcus aureus
2.5.1.7 the murZ gene is cloned and overexpressed in Escherichia coli with a C-terminal 6-His tag Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.7 fosfomycin despite low level expression during normal growth, murZ expression is strongly induced up to 6fold following exposure to inhibitors of peptidoglycan biosynthesis, minimum inhibitory concentration is 4 mg/ml; the naturally occurring antibiotic, which is an analogue of phosphoenolpyruvate, irreversibly inhibits the majority of MurA enzymes, fosfomycin minimum inhibitory concentration is 8 mg/ml, peptidoglycan content is reduced by approximately 25% following inactivation of murA Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.7 0.01148
-
 phosphoenolpyruvate 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus
2.5.1.7 0.01157
-
 phosphoenolpyruvate 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus
2.5.1.7 0.1239
-
 UDP-N-acetyl-D-glucosamine 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus
2.5.1.7 0.1686
-
 UDP-N-acetyl-D-glucosamine 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine Staphylococcus aureus
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 by using affinity chromatography Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-D-glucosamine
-
 Staphylococcus aureus phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 MurA
-
 Staphylococcus aureus
2.5.1.7 MurZ
-
 Staphylococcus aureus
2.5.1.7 UDP-GlcNAc enolpyruvyl transferase
-
 Staphylococcus aureus
2.5.1.7 UDP-N-acetylglucosamine enolpyruvyl transferase
-
 Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.7 0.76
-
 fosfomycin 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus
2.5.1.7 1.13
-
 fosfomycin 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.7 0.00136
-
 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus fosfomycin
2.5.1.7 0.00137
-
 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus fosfomycin

General Information

EC Number General Information Comment Organism
2.5.1.7 physiological function enzyme catalyzes the first committed step of peptidoglycan biosynthesis Staphylococcus aureus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.7 0.000005
-
 UDP-N-acetyl-D-glucosamine 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus
2.5.1.7 0.000009
-
 UDP-N-acetyl-D-glucosamine 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus
2.5.1.7 0.00007
-
 phosphoenolpyruvate 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus
2.5.1.7 0.0001
-
 phosphoenolpyruvate 50 mM HEPES, 250 mM methylthioguanosine, 0.1 U/ml purine-nucleoside phosphorylase, 100 mM dithiothreitol, pH 7.6 Staphylococcus aureus