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Literature summary extracted from

  • Kim, T.G.; Kwon, T.H.; Min, K.; Dong, M.S.; Park, Y.I.; Ban, C.
    Crystal structures of substrate and inhibitor complexes of ribose 5-phosphate isomerase A from Vibrio vulnificus YJ016 (2009), Mol. Cells, 27, 99-103.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6 gene rpiA, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) Vibrio vulnificus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.6 open form RpiA in complex with substrate ribose 5-phosphate, the closed form complexed with arabinose-5-phosphate, and the apo-RpiA, hanging drop vapor diffusion method, 0.002 ml of 30 mg/ml protein in 20 mM HEPES, pH 7.5, and 150 mM KCl are mixed with 0.002 ml of a reservoir solution containing 50 mM succinate, pH 4.1, 180 mM ammonium sulfate, and 8% PEG 4000, 3 days, 20°C, for complexed enzyme addition of 20 mM ligand, X-ray diffraction structure determination and analysis at 1.49-2.07 A resolution, molecular replacement Vibrio vulnificus

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.6 arabinose 5-phosphate RpiA binds arabinose 5-phosphate located at the opening of the active site, the sugar ring of the inhibitor interacts with the Asp4, Lys7, Ser30, Asp118, and Lys121 residues, the phosphate group of arabinose 5-phosphate interacts with two water molecules, W51 and W82, binding mode of inhibitor, overview Vibrio vulnificus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 45000
-
 recombinant detagged RpiA, dynamic light scattering and gel filtration Vibrio vulnificus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate Vibrio vulnificus
-
 D-ribulose 5-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Vibrio vulnificus Q7MHL9 gene rpiA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.6 recombinant His-tagged RpiA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography. The His-tag is cleaved off and removed, folowed by anion exchange chromatography and gel filtration Vibrio vulnificus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-ribose 5-phosphate
-
 Vibrio vulnificus D-ribulose 5-phosphate
-
 r
5.3.1.6 D-ribose 5-phosphate binding mode of substrate, overview Vibrio vulnificus D-ribulose 5-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.1.6 dimer 2 * 20000-25000, RpiA Vibrio vulnificus

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 ribose 5-phosphate isomerase A
-
 Vibrio vulnificus
5.3.1.6 RpiA
-
 Vibrio vulnificus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.6 Vibrio vulnificus isoelectric focusing
-
 4.77