EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.5.1 | overexpressed in Escherichia coli BL21 (DE3) strain using the pET-28a(+) expression vector | Oryza sativa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.5.1 | Oryza sativa | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.5.1 | glutathione + dehydroascorbate | - |
Oryza sativa | glutathione disulfide + ascorbate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.5.1 | OsDHAR | - |
Oryza sativa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.5.1 | additional information | - |
assay performed at room temperature | Oryza sativa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.5.1 | 7 | - |
assay at | Oryza sativa |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.5.1 | physiological function | OsDHAR transformed Escherichia coli BL21 cells show significantly higher DHAR activity and a lower level of reactive oxygen species than the Escherichia coli cells transformed by an empty vector. The DHAR-overexpressing Escherichia coli strain is more tolerant to oxidant- and heavy metalmediated stress conditions than the control Escherichia coli strain, suggesting that the overexpressed rice DHAR gene effectively functions in a prokaryotic system and provides protection to various oxidative stresses | Oryza sativa |