Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gusarova, G.A.; Dada, L.A.; Kelly, A.M.; Brodie, C.; Witters, L.A.; Chandel, N.S.; Sznajder, J.I.
    Alpha1-AMP-activated protein kinase regulates hypoxia-induced Na,K-ATPase endocytosis via direct phosphorylation of protein kinase C zeta (2009), Mol. Cell. Biol., 29, 3455-3464.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.31 O2 hypoxia leads to time-dependent AMPK activation in ATII cells. Maximal activation of AMPK after 10 min of 1.5% O2 exposure, whereas 3% O2 activates AMPK in a similar but slower manner. AMPK levels return to the baseline after 30 min of hypoxia exposure. Hypoxia-generated mitochondrial reactive oxygen species leads to the activation of the AMPK alpha1 isoform at Thr172. Hypoxia fails to activate AMPK in mitochondrion-deficient rho0-A549 cells Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.31 ATII cells infected with a hemagglutinin-tagged adenovirus carrying the dominant-negative mutant K45R of the AMPK alpha1 subunit or with an adenovirus expressing a constitutively active AMPK alpha variant or with an adenovirus expressing a constitutively active AMPK alpha variant in which Thr172 is replaced with aspartate in the truncated AMPK alpha subunit, comprising residues 1 to 312. A549 cells transfected with AMPK alpha1 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.31 K45R dominant-negative, kinase-dead mutant of the AMPK alpha1 subunit Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.31 6-[4-(2-piperidin-1-yl-ethoxy)-phenyl]-3-pyridin-4-yl-pyrrazolo[1,5-a]-pyrimidine compound C, hypoxia-induced PKCzeta translocation to the plasma membrane and phosphorylation at Thr410 is prevented by pharmacological inhibition of AMPK Homo sapiens
2.7.11.31 additional information overexpression of reactive oxygen species scavenger catalase prevents hypoxia-induced AMPK activation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.31 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.31 alveolar epithelium
-
 Homo sapiens
-
2.7.11.31 additional information ATII cell Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.31 ATP + acetyl-CoA carboxylase AMPK alpha phosphorylates at Ser79 Homo sapiens ADP + [acetyl-CoA carboxylase]phosphate
-
 ?
2.7.11.31 ATP + PKZeta AMPK alpha phosphorylates PKZeta on residue Thr410 within the PKCzeta activation loop Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.31 AMP-activated protein kinase
-
 Homo sapiens
2.7.11.31 AMPK
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.11.31 physiological function activation of AMPK alpha is necessary for hypoxia-induced AMPK-PKCzeta binding in alveolar epithelial cells. Overexpression of a dominant-negative AMPK alpha subunit construct prevents hypoxia-induced endocytosis of Na,K-ATPase, hypoxia-induced PKCzeta translocation to the plasma membrane and phosphorylation at Thr410 Homo sapiens