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Literature summary extracted from

  • Gracheva, E.V.; Samovilova, N.N.; Golovanova, N.K.; Kashirina, S.V.; Shevelev, A.; Rybalkin, I.; Gurskaya, T.; Vlasik, T.N.; Andreeva, E.R.; Prokazova, N.V.
    Enhancing of GM3 synthase expression during differentiation of human blood monocytes into macrophages as in vitro model of GM3 accumulation in atherosclerotic lesion (2009), Mol. Cell. Biochem., 330, 121-129.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.9 DL-threo-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol inhibitor of ganglioside synthesis, depletion of GM3 synthesis in cultured monocyte-derived macrophages, delays acquisition of CD206 antigen, prevents loss of CD163 antigen and enhances anti-inflammatory cytokine (CCL18) secretion Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.9 0.101
-
 lactosylceramide
-
 Homo sapiens
2.4.3.9 0.18
-
 CMP-N-acetylneuraminate
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.9 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.3.9 by immunoaffinity chromatography, to apparent homogeneity, 3600fold, with a yield of 19% Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.9 aorta atherosclerotic and normal aorta Homo sapiens
-
2.4.3.9 macrophage differentiated monocyte-derived macrophages Homo sapiens
-
2.4.3.9 monocyte
-
 Homo sapiens
-
2.4.3.9 peripheral blood mononuclear cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.9 0.000000167
-
 homogenate Homo sapiens
2.4.3.9 0.0006048
-
 3600fold purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.9 CMP-N-acetylneuraminate + asialoGM1 27.7% relative activity to lactosylceramide Homo sapiens ?
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + galactosylceramide 30% relative activity to lactosylceramide Homo sapiens ?
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + GM3 4.5% relative activity to lactosylceramide Homo sapiens ?
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + lactosylceramide 100% relative activity Homo sapiens ?
-
 ?
2.4.3.9 additional information no acitvity with GM1 or asialofetuin as acceptor Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.3.9 CMP-N-acetylneuraminic acid:lactosylceramide alpha2,3-sialyltransferase
-
 Homo sapiens
2.4.3.9 GM3 synthase
-
 Homo sapiens
2.4.3.9 ST3Gal V
-
 Homo sapiens

Expression

EC Number Organism Comment Expression
2.4.3.9 Homo sapiens GM3 synthase mRNA levels are significantly higher in differentiated monocyte-derived macrophages compared to monocytes and in atherosclerotic aorta compared to normal aorta up