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Literature summary extracted from

  • Chavez-Aviles, M.; Diaz-Perez, A.L.; Campos-Garcia, J.
    The bifunctional role of LiuE from Pseudomonas aeruginosa, displays additionally HIHG-CoA lyase enzymatic activity (2010), Mol. Biol. Rep., 37, 1787-1791.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.4 expressed as His-tagged recombinant protein in Escherichia coli pTrcAtuE cells Pseudomonas aeruginosa
4.1.3.26 His-tagged protein expressed in Escherichia coli JM101 Pseudomonas aeruginosa PAO1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.4 33000
-
 LiuE His-tagged protein, SDS-PAGE Pseudomonas aeruginosa
4.1.3.26 33000
-
 x * 33000, SDS-PAGE Pseudomonas aeruginosa PAO1

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.4 Pseudomonas aeruginosa
-
-
-
4.1.3.4 Pseudomonas aeruginosa PAO1SM
-
-
-
4.1.3.26 Pseudomonas aeruginosa PAO1 Q9I2A0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.26 by affinity chromatography, more than 98% purified Pseudomonas aeruginosa PAO1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA
-
 Pseudomonas aeruginosa Acetyl-CoA + acetoacetate
-
 ?
4.1.3.4 (S)-3-Hydroxy-3-methylglutaryl-CoA
-
 Pseudomonas aeruginosa PAO1SM Acetyl-CoA + acetoacetate
-
 ?
4.1.3.26 3-hydroxy-3-isohexenylglutaryl-CoA
-
 Pseudomonas aeruginosa PAO1 7-methyl-3-oxo-6-octenyl-CoA + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.26 ? x * 33000, SDS-PAGE Pseudomonas aeruginosa PAO1

Synonyms

EC Number Synonyms Comment Organism
4.1.3.4 3-hydroxy-3-methylglutaryl CoA lyase
-
 Pseudomonas aeruginosa
4.1.3.4 HMG-CoA lyase
-
 Pseudomonas aeruginosa
4.1.3.4 LiuE protein in addition to show HMG-CoA lyase activity, LiuE also displays 3-hydroxy-3-isohexenylglutaryl-CoA lyase activity Pseudomonas aeruginosa
4.1.3.26 3-hydroxy-3-isohexenylglutaryl-CoA lyase
-
 Pseudomonas aeruginosa PAO1
4.1.3.26 HIHG-CoA lyase
-
 Pseudomonas aeruginosa PAO1
4.1.3.26 LiuE
-
 Pseudomonas aeruginosa PAO1

General Information

EC Number General Information Comment Organism
4.1.3.26 metabolism LiuE displays a bifunctional role in both the leucine/isovalerate and acyclic terpenes catabolic pathways, LiuE shows enzymatic activity of HMG-CoA lyase and also HIHG-CoA lyase activity Pseudomonas aeruginosa PAO1