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Literature summary extracted from

  • Domin, N.; Wilson, D.; Brock, M.
    Methylcitrate cycle activation during adaptation of Fusarium solani and Fusarium verticillioides to propionyl-CoA-generating carbon sources (2009), Microbiology, 155, 3903-3912.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.5 into the vector pJET1.2 for sequencing, into a modified pET43.1a vector for expression in Escherichia coli BL21DE3 Rosetta 2 cells Fusarium solani
2.3.3.5 into the vector pJET1.2 for sequencing, into a modified pET43.1a vector for expression in Escherichia coli BL21DE3 Rosetta 2 cells Fusarium verticillioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.5 0.00126
-
 acetyl-CoA
-
 Fusarium solani
2.3.3.5 0.00188
-
 propionyl-CoA
-
 Fusarium verticillioides
2.3.3.5 0.00194
-
 propionyl-CoA
-
 Fusarium solani
2.3.3.5 0.00258
-
 oxaloacetate propionyl-CoA Fusarium solani
2.3.3.5 0.00271
-
 acetyl-CoA
-
 Fusarium verticillioides
2.3.3.5 0.00281
-
 oxaloacetate propionyl-CoA Fusarium verticillioides
2.3.3.5 0.00385
-
 oxaloacetate acetyl-CoA Fusarium solani
2.3.3.5 0.00964
-
 oxaloacetate acetyl-CoA Fusarium verticillioides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.5 49000
-
 determined by SDS-PAGE Fusarium solani
2.3.3.5 49000
-
 determined by SDS-PAGE Fusarium verticillioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.5 propionyl-CoA + H2O + oxaloacetate Fusarium solani
-
 (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?
2.3.3.5 propionyl-CoA + H2O + oxaloacetate Fusarium verticillioides
-
 (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Fusarium solani
-
-
-
2.3.3.5 Fusarium verticillioides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.5 on a gravity-flow Ni-NTA agarose column Fusarium solani
2.3.3.5 on a gravity-flow Ni-NTA agarose column Fusarium verticillioides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.5 15.31
-
 propionyl-CoA Fusarium verticillioides
2.3.3.5 19.05
-
 acetyl-CoA Fusarium solani
2.3.3.5 19.55
-
 propionyl-CoA Fusarium solani
2.3.3.5 36.6
-
 acetyl-CoA Fusarium verticillioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 acetyl-CoA + H2O + oxaloacetate
-
 Fusarium verticillioides citrate + CoA
-
 ?
2.3.3.5 acetyl-CoA + H2O + oxaloacetate
-
 Fusarium solani ? + CoA
-
 ?
2.3.3.5 propionyl-CoA + H2O + oxaloacetate
-
 Fusarium solani (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?
2.3.3.5 propionyl-CoA + H2O + oxaloacetate
-
 Fusarium verticillioides (2R,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.5 Methylcitrate synthase
-
 Fusarium solani
2.3.3.5 Methylcitrate synthase
-
 Fusarium verticillioides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.3.5 49 54 maximum activity Fusarium solani
2.3.3.5 49 54 maximum activity Fusarium verticillioides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.5 12.5
-
 propionyl-CoA
-
 Fusarium verticillioides
2.3.3.5 15.5
-
 acetyl-CoA
-
 Fusarium solani
2.3.3.5 15.9
-
 propionyl-CoA
-
 Fusarium solani
2.3.3.5 29.9
-
 acetyl-CoA
-
 Fusarium verticillioides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.5 8 9 maximum activity Fusarium solani
2.3.3.5 8 9 maximum activity Fusarium verticillioides

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.3.5 CoA
-
 Fusarium solani
2.3.3.5 CoA
-
 Fusarium verticillioides

General Information

EC Number General Information Comment Organism
2.3.3.5 physiological function propionyl-CoA detoxification Fusarium solani
2.3.3.5 physiological function propionyl-CoA detoxification Fusarium verticillioides