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Literature summary extracted from

  • Wiemann, L.O.; Buthe, A.; Klein, M.; van den Wittenboer, A.; Daehne, L.; Ansorge-Schumacher, M.B.
    Encapsulation of synthetically valuable biocatalysts into polyelectrolyte multilayer systems (2009), Langmuir, 25, 618-623.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.38 expressed in Escherichia coli strain SG13009 containing plasmid pBALhis6 Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.38 additional information BAL encapsulated in the standard polyelectrolyte multilayer-system retains only about 2% of its native specific activity. An activity decrease of 50% is observed as a result of the deposition of every additional polyelectrolyte layer on the enzyme or when free BAL is dissolved in an aqueous system. Adsorption of two poly(allylamine) hydrochloride/poly(styrene sulfonate) sodium salt double layers on whole cells containing BAL reduces their catalytic activity to 0.01% of the initial value Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Mg2+ dependent on Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.38 58000
-
 4 * 58000, SDS-PAGE Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 Pseudomonas fluorescens
-
 biovar I
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.38 0.22
-
 enzyme-polyelectrolyte multilayer capsules Pseudomonas fluorescens
4.1.2.38 10.9
-
 native enzyme Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 benzaldehyde + benzaldehyde
-
 Pseudomonas fluorescens benzoin
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.38 tetramer 4 * 58000, SDS-PAGE Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.38 BAL
-
 Pseudomonas fluorescens
4.1.2.38 benzaldehyde lyase
-
 Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.38 7.4
-
-
 Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate
-
 Pseudomonas fluorescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.2.38 Pseudomonas fluorescens
-
-
 4.6