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Literature summary extracted from

  • Fujii, T.; Masai, M.; Misawa, H.; Okuda, T.; Takada-Takatori, Y.; Moriwaki, Y.; Haga, T.; Kawashima, K.
    Acetylcholine synthesis and release in NIH3T3 cells coexpressing the high-affinity choline transporter and choline acetyltransferase (2009), J. Neurosci. Res., 87, 3024-3032.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.6 choline extracellular choline increases acetylcholine contents in the cell Mus musculus
2.3.1.6 high-affinity choline transporter i.e. CHT1, plays a key role in acetylcholine synthesis and choline uptake, CHT1 inhibition also inhibits acetylcholine formation, overview Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.6 stable expression of the enzyme in NIH-3T3 fibroblasts, expression of ChAT and high-affinity choline transporter CHT1 in NIH-3T3 cells giving NIH3T3ChAT 112-1 cells leading to increased binding of the CHT1 inhibitor hemicholinium-3 and greater choline uptake and acetylcholine synthesis compared to NIH3T3ChAT 103-1 cells, a CHT1-negative control cell line Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.6 acetyl-CoA + choline Mus musculus choline taken up by OCTs or derived from the plasma membrane is also utilized for acetylcholine synthesis in both cholinergic neurons and nonneuronal cholinergic cells, such as lymphocytes CoA + O-acetylcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.6 Mus musculus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.6 lymphocyte
-
 Mus musculus
-
2.3.1.6 neuron
-
 Mus musculus
-
2.3.1.6 neuron cholinergic Mus musculus
-
2.3.1.6 non-neuronal cell non-neuronal cholinergic cell Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.6 acetyl-CoA + choline
-
 Mus musculus CoA + O-acetylcholine
-
 ?
2.3.1.6 acetyl-CoA + choline choline taken up by OCTs or derived from the plasma membrane is also utilized for acetylcholine synthesis in both cholinergic neurons and nonneuronal cholinergic cells, such as lymphocytes Mus musculus CoA + O-acetylcholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.6 ChAT
-
 Mus musculus
2.3.1.6 choline acetyltransferase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.6 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.6 7.4
-
 assay at Mus musculus