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Literature summary extracted from

  • Okuyama, R.; Marshall, S.
    UDP-N-acetylglucosaminyl transferase (OGT) in brain tissue: temperature sensitivity and subcellular distribution of cytosolic and nuclear enzyme (2003), J. Neurochem., 86, 1271-1280.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.255 ATP has a much lower affinity for OGT Rattus norvegicus
2.4.1.255 KCl lowers OGT activity by reducing enzyme affinity for UDP-GlcNAc Rattus norvegicus
2.4.1.255 NaCl
-
 Rattus norvegicus
2.4.1.255 NaH2PO4
-
 Rattus norvegicus
2.4.1.255 UDP-galactose much lower affinity for OGT compared with UDP-GlcNAc Rattus norvegicus
2.4.1.255 UDP-GlcNAc
-
 Rattus norvegicus
2.4.1.255 UDP-glucose much lower affinity for OGT compared with UDP-GlcNAc Rattus norvegicus
2.4.1.255 UTP has a high affinity for OGT Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.255 cytosol
-
 Rattus norvegicus 5829
-
2.4.1.255 nucleus
-
 Rattus norvegicus 5634
-

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.255 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.255 immunopurified Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.255 adipose
-
 Rattus norvegicus
-
2.4.1.255 brain cytosolic OGT activity is 10times more abundant in brain tissue compared with muscle, adipose, heart, and liver tissue Rattus norvegicus
-
2.4.1.255 heart
-
 Rattus norvegicus
-
2.4.1.255 liver
-
 Rattus norvegicus
-
2.4.1.255 muscle
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.255 UDP-GlcNAc + beta-amyloid associated protein
-
 Rattus norvegicus GlcNAc-beta-amyloid associated protein + UDP
-
 ?
2.4.1.255 UDP-GlcNAc + tau protein
-
 Rattus norvegicus GlcNAc-tau protein + UDP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.255 O-GlcNAc transferase
-
 Rattus norvegicus
2.4.1.255 OGT
-
 Rattus norvegicus
2.4.1.255 UDP-N-acetylglucosaminyl transferase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.255 24 27 a slight loss in enzyme activity is seen at 30°C, almost a complete loss of activity occurs at 37°C. At 33°C there is a 45% decrease in OGT activity Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.255 additional information
-
 cytosolic OGT activity is stable at 24°C but was rapidly inactivated at 37°C (t1/2 = 20 min). Nuclear OGT is not inactivated at 37°C Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.1.255 0.00025
-
-
 Rattus norvegicus UDP-GlcNAc
2.4.1.255 0.0005
-
-
 Rattus norvegicus UTP
2.4.1.255 0.0042
-
 much lower affinity for OGT compared with UDP-GlcNAc Rattus norvegicus UDP-glucose
2.4.1.255 0.013
-
 much lower affinity for OGT compared with UDP-GlcNAc Rattus norvegicus UDP-galactose
2.4.1.255 0.86
-
-
 Rattus norvegicus ATP
2.4.1.255 4
-
 about Rattus norvegicus NaCl
2.4.1.255 45
-
-
 Rattus norvegicus NaH2PO4
2.4.1.255 50
-
 about Rattus norvegicus KCl