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Literature summary extracted from

  • Kim, S.K.; Ahn, K.H.; Jeon, H.J.; Lee, D.H.; Jung, S.Y.; Jung, K.M.; Kim, D.K.
    Purification of neutral sphingomyelinase 2 from bovine brain and its calcium-dependent activation (2010), J. Neurochem., 112, 1088-1097.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 phosphatidylserine
-
Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expressed in COS-7 cells Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 GW4869 inhibited by 0.001-0.1 mM Bos taurus
3.1.4.12 additional information not inhibited by fumonisin B1 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.0203
-
sphingomyelin in 100 mM Tris-HCl (pH 7.5), at 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 membrane
-
Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Ca2+ activated by micromolar concentrations of Ca2+ (0.0001 or 0.00001 mM) under Mg2+-free conditions, ceramide generation is dependent upon the calcium ionophore A23187, nSMase is not activated by Ca2+ cations in the presence of 1 mM Mg2+ Bos taurus
3.1.4.12 additional information Cu2+, Zn2+, and Ni2+ are unable to stimulate activity, nSMase is not activated by Ca2+ cations in the presence of 1 mM Mg2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.12 75000
-
SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 ammonium sulfate precipitation, DEAE-cellulose column chromatography, butyl-Toyopearl column chromatography, DEAE-5PW HPLC column chromatography, Sephacryl S-300 gel filtration, Mono Q column chromatography, Heparin Hi-TRAP column chromatography, and Superose 12 gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 0.000114
-
after 1fold purification, at 37°C Bos taurus
3.1.4.12 0.9334
-
after 8183.3fold purification, at 37°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
Bos taurus N-acylsphingosine + choline phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 neutral sphingomyelinase 2
-
Bos taurus
3.1.4.12 nSMase2
-
Bos taurus