EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | phosphatidylserine | - |
Bos taurus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.12 | expressed in COS-7 cells | Bos taurus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | GW4869 | inhibited by 0.001-0.1 mM | Bos taurus | |
3.1.4.12 | additional information | not inhibited by fumonisin B1 | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.4.12 | 0.0203 | - |
sphingomyelin | in 100 mM Tris-HCl (pH 7.5), at 37°C | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.12 | membrane | - |
Bos taurus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.12 | Ca2+ | activated by micromolar concentrations of Ca2+ (0.0001 or 0.00001 mM) under Mg2+-free conditions, ceramide generation is dependent upon the calcium ionophore A23187, nSMase is not activated by Ca2+ cations in the presence of 1 mM Mg2+ | Bos taurus | |
3.1.4.12 | additional information | Cu2+, Zn2+, and Ni2+ are unable to stimulate activity, nSMase is not activated by Ca2+ cations in the presence of 1 mM Mg2+ | Bos taurus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 75000 | - |
SDS-PAGE | Bos taurus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.12 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.12 | ammonium sulfate precipitation, DEAE-cellulose column chromatography, butyl-Toyopearl column chromatography, DEAE-5PW HPLC column chromatography, Sephacryl S-300 gel filtration, Mono Q column chromatography, Heparin Hi-TRAP column chromatography, and Superose 12 gel filtration | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.12 | brain | - |
Bos taurus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.12 | 0.000114 | - |
after 1fold purification, at 37°C | Bos taurus |
3.1.4.12 | 0.9334 | - |
after 8183.3fold purification, at 37°C | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.12 | sphingomyelin + H2O | - |
Bos taurus | N-acylsphingosine + choline phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.4.12 | neutral sphingomyelinase 2 | - |
Bos taurus |
3.1.4.12 | nSMase2 | - |
Bos taurus |