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Literature summary extracted from

  • Yang, Y.; Kang, Y.; Kim, D.; Lee, T.; Park, S.; Lee, K.; Yoo, D.; Liou, K.; Lee, H.; Sohng, J.; Kim, B.
    One-pot enzymatic synthesis of deoxy-thymidine-diphosphate (TDP)-2-deoxy-alpha-D-glucose using phosphomannomutase (2010), J. Mol. Catal. B, 62, 282-287.
No PubMed abstract available

Application

EC Number Application Comment Organism
5.4.2.8 biotechnology enzymatic synthesis of TMP and 2-deoxy-6-phosphate glucose can successfully produce large amount of TDP-2-deoxy-glucose in one-pot by employing phosphomannomutase Escherichia coli K-12

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.8 into the vector pET24ma for transformation of Escherichia coli BL21DE3 cells Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.8 alpha-D-mannose 1-phosphate Escherichia coli K-12
-
 D-mannose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.8 Escherichia coli K-12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.8 cell extracts are purified by His-tag agarose beads Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.8 2-deoxy-D-glucose 6-phosphate
-
 Escherichia coli K-12 2-deoxy-D-glucose 1-phosphate
-
 ?
5.4.2.8 alpha-D-mannose 1-phosphate
-
 Escherichia coli K-12 D-mannose 6-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.2.8 phosphomannomutase
-
 Escherichia coli K-12
5.4.2.8 PMM
-
 Escherichia coli K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.2.8 25
-
 kinetic assay Escherichia coli K-12
5.4.2.8 37
-
 activity assay Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.2.8 7.5
-
 activity assay Escherichia coli K-12
5.4.2.8 7.8
-
 kinetic assay Escherichia coli K-12