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Literature summary extracted from

  • Würges, K.; Petrusevska, K.; Serci, S.; Wilhelm, S.; Wandrey, C.; Seidel-Morgenstern, A.; Elsner, M.; Lütz, S.
    Enzyme-assisted physicochemical enantioseparation processes: Part I. Production and characterization of a recombinant amino acid racemase (2009), J. Mol. Catal. B, 58, 10-16.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.1.10 over-expressed in Escherichia coli Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.10 2 3 L-Met crude lyophilisates, 25°C, pH 7, Vmax: 2150 micromol/min/g Pseudomonas putida
5.1.1.10 2 3 D-Met crude lyophilisates, 30°C, pH 7, Vmax: 2075 micromol/min/g Pseudomonas putida
5.1.1.10 18
-
 D-Asn pure lyophilisates, 20°C, pH 7, Vmax: 770 micromol/min/g Pseudomonas putida
5.1.1.10 20
-
 L-Met crude lyophilisates, 25°C, pH 8, Vmax: 2951 micromol/min/g Pseudomonas putida
5.1.1.10 22
-
 D-Met crude lyophilisates, 25°C, pH 7, Vmax: 1892 micromol/min/g Pseudomonas putida
5.1.1.10 25
-
 D-Met crude lyophilisates, 20°C, pH 7, Vmax: 1696 micromol/min/g Pseudomonas putida
5.1.1.10 28
-
 D-Met crude lyophilisates, 25°C, pH 8, Vmax: 3627 micromol/min/g Pseudomonas putida
5.1.1.10 30
-
 L-Met crude lyophilisates, 25°C, pH 6, Vmax: 1174 micromol/min/g Pseudomonas putida
5.1.1.10 30
-
 L-Met crude lyophilisates, 30°C, pH 7, Vmax: 2296 micromol/min/g Pseudomonas putida
5.1.1.10 30
-
 D-Met crude lyophilisates, 35°C, pH 7, Vmax: 2512 micromol/min/g Pseudomonas putida
5.1.1.10 30
-
 L-Asn pure lyophilisates, 20°C, pH 7, Vmax: 1626 micromol/min/g Pseudomonas putida
5.1.1.10 31
-
 L-Met crude lyophilisates, 20°C, pH 7, Vmax: 1889 micromol/min/g Pseudomonas putida
5.1.1.10 32
-
 D-Met crude lyophilisates, 40°C, pH 7, Vmax: 2668 micromol/min/g Pseudomonas putida
5.1.1.10 35
-
 L-Met crude lyophilisates, 40°C, pH 7, Vmax: 2847 micromol/min/g Pseudomonas putida
5.1.1.10 39
-
 L-Met crude lyophilisates, 35°C, pH 7, Vmax: 2833 micromol/min/g Pseudomonas putida
5.1.1.10 42
-
 D-Met crude lyophilisates, 25°C, pH 6, Vmax: 1160 micromol/min/g Pseudomonas putida
5.1.1.10 47
-
 L-Asn pure lyophilisates, 25°C, pH 7, Vmax: 2427 micromol/min/g Pseudomonas putida
5.1.1.10 50
-
 D-Asn pure lyophilisates, 25°C, pH 7, Vmax: 1299 micromol/min/g Pseudomonas putida
5.1.1.10 62
-
 D-Asn pure lyophilisates, 30°C, pH 7, Vmax: 1881 micromol/min/g Pseudomonas putida
5.1.1.10 66
-
 D-Asn pure lyophilisates, 35°C, pH 7, Vmax: 2585 micromol/min/g Pseudomonas putida
5.1.1.10 76
-
 L-Asn pure lyophilisates, 30°C, pH 7, Vmax: 3930 micromol/min/g Pseudomonas putida
5.1.1.10 101
-
 L-Asn pure lyophilisates, 35°C, pH 7, Vmax: 5683 micromol/min/g Pseudomonas putida
5.1.1.10 103
-
 D-Asn pure lyophilisates, 40°C, pH 7, Vmax: 4032 micromol/min/g Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.10 45000
-
 SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.10 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.10 two enzyme lyophilisates of different purity are obtained from which the crude is sufficient for the racemization of methionine and the pure is used for asparagine Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.10 D-Asn
-
 Pseudomonas putida L-Asn
-
 r
5.1.1.10 D-Met
-
 Pseudomonas putida L-Met
-
 r
5.1.1.10 L-Asn
-
 Pseudomonas putida D-Asn
-
 r
5.1.1.10 L-Met
-
 Pseudomonas putida D-Met
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.1.10 homodimer 2 * 45000 Da, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
5.1.1.10 amino acid racemase
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.10 pyridoxal 5'-phosphate
-
 Pseudomonas putida