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Literature summary extracted from

  • Huerta, C.; Borek, D.; Machius, M.; Grishin, N.V.; Zhang, H.
    Structure and mechanism of a eukaryotic FMN adenylyltransferase (2009), J. Mol. Biol., 389, 388-400.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.2 His-tagged version expressed in Escherichia coli BL21(DE3) Nakaseomyces glabratus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.2
-
 Nakaseomyces glabratus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.2 R297A involved in substrate binding Nakaseomyces glabratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.2 additional information
-
 additional information R297A mutant protein: increased apparent KM-values for ATP and FMN by about 5 and 3times, respectively, compared to the wild-type enzyme Nakaseomyces glabratus
2.7.7.2 0.00076
-
 FMN
-
 Nakaseomyces glabratus
2.7.7.2 0.0107
-
 ATP
-
 Nakaseomyces glabratus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.2 Mg2+
-
 Nakaseomyces glabratus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.2 Nakaseomyces glabratus Q6FNA9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.2 Ni-Sepharose affinity chromatography, anion exchange chromatography, hydrophobic interaction chromatography, gel filtration Nakaseomyces glabratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.2 FMN + ATP
-
 Nakaseomyces glabratus FAD + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.2 monomer crystal structure, gel filtration Nakaseomyces glabratus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.2 FMN adenylyltransferase
-
 Nakaseomyces glabratus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.2 0.087
-
 ATP
-
 Nakaseomyces glabratus
2.7.7.2 0.087
-
 FMN
-
 Nakaseomyces glabratus