EC Number | Cloned (Comment) | Organism |
---|---|---|
1.20.4.4 | C10S/C15A/C89L mutant and C10S/C15A/C82S mutant expressed in Escherichia coli | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.20.4.4 | C10S/C15A/C82S | mutant, determination of the redox potential of the Cys82-Cys89 redox couple, thioredoxin is unable to reduce the Cys10-Cys15 disulfide in oxidized ArsC C82S | Staphylococcus aureus |
1.20.4.4 | C10S/C15A/C89L | mutant, determination of the redox potential of the Cys82-Cys89 redox couple | Staphylococcus aureus |
1.20.4.4 | additional information | ArsC triple mutants and the Trx C32S mutant formate a ArsC-TrxC32S complex | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.4.4 | 0.033 | - |
thioredoxin | - |
Staphylococcus aureus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.20.4.4 | 14800 | - |
reduced C82S mutant | Staphylococcus aureus |
1.20.4.4 | 14810 | - |
reducted wild-type | Staphylococcus aureus |
1.20.4.4 | 14820 | - |
reduced C89L mutant | Staphylococcus aureus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.20.4.4 | Staphylococcus aureus | P0A006 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.20.4.4 | C10S/C15A/C89L mutant and C10S/C15A/C82S mutant purified by Ni2+-NTA Superflow | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.20.4.4 | arsenate + thioredoxin | - |
Staphylococcus aureus | arsenite + thioredoxin disulfide + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.20.4.4 | ArsC | - |
Staphylococcus aureus |
1.20.4.4 | pI258 arsenate reductase | - |
Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.4.4 | 1.9 | - |
thioredoxin | - |
Staphylococcus aureus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.20.4.4 | thioredoxin | interaction between thioredoxin and the enzyme pI258 arsenate reductase. Thioredoxin converts oxidized ArsC back towards its initial reduced state | Staphylococcus aureus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.20.4.4 | 57.6 | - |
thioredoxin | - |
Staphylococcus aureus |