Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gangjee, A.; Li, W.; Kisliuk, R.L.; Cody, V.; Pace, J.; Piraino, J.; Makin, J.
    Design, synthesis, and X-ray crystal structure of classical and nonclassical 2-amino-4-oxo-5-substituted-6-ethylthieno[2,3-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors and as potential antitumor agents (2009), J. Med. Chem., 52, 4892-4902.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3 expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.3 enzyme mutant Q35S/N64S in complex with NADPH and inhibitor N-(4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid, and enzyme mutant Q35K in complex with NADPH and inhibitor N-{4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl}-L-glutamic acid, X-ray diffraction structure determination and analysis at 1.2-1.5 A resolution Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]-pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]-pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]-pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d ]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
1.5.1.3 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
1.5.1.3 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
1.5.1.3 methotrexate
-
 Escherichia coli
1.5.1.3 methotrexate
-
 Homo sapiens
1.5.1.3 methotrexate
-
 Toxoplasma gondii
1.5.1.3 N-(4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
-
 Escherichia coli
1.5.1.3 N-(4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
-
 Homo sapiens
1.5.1.3 N-(4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
-
 Toxoplasma gondii
1.5.1.3 N-(4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
-
 Escherichia coli
1.5.1.3 N-(4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
-
 Homo sapiens
1.5.1.3 N-(4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
-
 Toxoplasma gondii
1.5.1.3 nolatrexed
-
 Escherichia coli
1.5.1.3 nolatrexed
-
 Homo sapiens
1.5.1.3 nolatrexed
-
 Toxoplasma gondii
1.5.1.3 pemetrexed
-
 Escherichia coli
1.5.1.3 pemetrexed
-
 Homo sapiens
1.5.1.3 pemetrexed
-
 Toxoplasma gondii
1.5.1.3 permetrexed
-
 Escherichia coli
1.5.1.3 permetrexed
-
 Homo sapiens
1.5.1.3 permetrexed
-
 Toxoplasma gondii
1.5.1.3 piritrexim
-
 Escherichia coli
1.5.1.3 piritrexim
-
 Homo sapiens
1.5.1.3 piritrexim
-
 Toxoplasma gondii
1.5.1.3 plevitrexed
-
 Escherichia coli
1.5.1.3 plevitrexed
-
 Homo sapiens
1.5.1.3 plevitrexed
-
 Toxoplasma gondii
1.5.1.3 raltitrexed
-
 Escherichia coli
1.5.1.3 raltitrexed
-
 Homo sapiens
1.5.1.3 raltitrexed
-
 Toxoplasma gondii
1.5.1.3 trimethoprim
-
 Escherichia coli
1.5.1.3 trimethoprim
-
 Homo sapiens
1.5.1.3 trimethoprim
-
 Toxoplasma gondii
1.5.1.3 trimetrexate
-
 Escherichia coli
1.5.1.3 trimetrexate
-
 Homo sapiens
1.5.1.3 trimetrexate
-
 Toxoplasma gondii
2.1.1.45 10-propargyl-5,8-dideazafolate
-
 Escherichia coli
2.1.1.45 10-propargyl-5,8-dideazafolate
-
 Homo sapiens
2.1.1.45 10-propargyl-5,8-dideazafolate
-
 Toxoplasma gondii
2.1.1.45 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]-pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]-pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]-pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Escherichia coli
2.1.1.45 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Homo sapiens
2.1.1.45 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
-
 Toxoplasma gondii
2.1.1.45 4-amino-N10-methylfolic acid
-
 Escherichia coli
2.1.1.45 4-amino-N10-methylfolic acid
-
 Homo sapiens
2.1.1.45 4-amino-N10-methylfolic acid
-
 Toxoplasma gondii
2.1.1.45 methotrexate
-
 Escherichia coli
2.1.1.45 methotrexate
-
 Homo sapiens
2.1.1.45 methotrexate
-
 Toxoplasma gondii
2.1.1.45 N-[4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)thio]benzoyl]-L-glutamic acid
-
 Escherichia coli
2.1.1.45 N-[4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)thio]benzoyl]-L-glutamic acid
-
 Homo sapiens
2.1.1.45 N-[4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)thio]benzoyl]-L-glutamic acid
-
 Toxoplasma gondii
2.1.1.45 N-[4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)sulfanyl]benzoyl]-L-glutamic acid potent inhibitor of thymidylate synthase Escherichia coli
2.1.1.45 N-[4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)sulfanyl]benzoyl]-L-glutamic acid potent inhibitor of thymidylate synthase Homo sapiens
2.1.1.45 N-[4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)sulfanyl]benzoyl]-L-glutamic acid potent inhibitor of thymidylate synthase Toxoplasma gondii
2.1.1.45 pemetrexed
-
 Escherichia coli
2.1.1.45 pemetrexed
-
 Homo sapiens
2.1.1.45 pemetrexed
-
 Toxoplasma gondii
2.1.1.45 piritrexim
-
 Escherichia coli
2.1.1.45 piritrexim
-
 Homo sapiens
2.1.1.45 piritrexim
-
 Toxoplasma gondii
2.1.1.45 plevitrexed
-
 Escherichia coli
2.1.1.45 plevitrexed
-
 Homo sapiens
2.1.1.45 plevitrexed
-
 Toxoplasma gondii
2.1.1.45 raltitrexed
-
 Escherichia coli
2.1.1.45 raltitrexed
-
 Homo sapiens
2.1.1.45 raltitrexed
-
 Toxoplasma gondii
2.1.1.45 trimethoprim
-
 Escherichia coli
2.1.1.45 trimethoprim
-
 Homo sapiens
2.1.1.45 trimethoprim
-
 Toxoplasma gondii
2.1.1.45 trimetrexate
-
 Escherichia coli
2.1.1.45 trimetrexate
-
 Homo sapiens
2.1.1.45 trimetrexate
-
 Toxoplasma gondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Escherichia coli
-
 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Toxoplasma gondii
-
 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Homo sapiens
-
 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli
-
-
-
1.5.1.3 Homo sapiens P00374
-
-
1.5.1.3 Toxoplasma gondii
-
-
-
2.1.1.45 Escherichia coli
-
-
-
2.1.1.45 Homo sapiens
-
-
-
2.1.1.45 Toxoplasma gondii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Toxoplasma gondii 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
 Homo sapiens 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Escherichia coli dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Toxoplasma gondii dihydrofolate + dTMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 DHFR
-
 Escherichia coli
1.5.1.3 DHFR
-
 Toxoplasma gondii
1.5.1.3 DHFR
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 37
-
 assay at Escherichia coli
1.5.1.3 37
-
 assay at Toxoplasma gondii
1.5.1.3 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 7
-
 assay at Escherichia coli
1.5.1.3 7
-
 assay at Toxoplasma gondii
1.5.1.3 7
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Escherichia coli
1.5.1.3 NADPH
-
 Toxoplasma gondii
1.5.1.3 NADPH
-
 Homo sapiens
2.1.1.45 N5,N10-methylenetetrahydrofolate
-
 Escherichia coli
2.1.1.45 N5,N10-methylenetetrahydrofolate
-
 Homo sapiens
2.1.1.45 N5,N10-methylenetetrahydrofolate
-
 Toxoplasma gondii

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.5.1.3 0.0000000088
-
 pH 7.0, 37°C Escherichia coli methotrexate
1.5.1.3 0.00000001
-
 pH 7.0, 37°C Escherichia coli trimethoprim
1.5.1.3 0.000000019
-
 pH 7.0, 37°C Homo sapiens N-(4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
1.5.1.3 0.00000002
-
 pH 7.0, 37°C Homo sapiens methotrexate
1.5.1.3 0.00000002
-
 pH 7.0, 37°C Homo sapiens N-(4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
1.5.1.3 0.0000002
-
 pH 7.0, 37°C Escherichia coli N-(4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
1.5.1.3 0.00000026
-
 pH 7.0, 37°C Homo sapiens 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.00000026
-
 pH 7.0, 37°C Homo sapiens 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.00000028
-
 pH 7.0, 37°C Homo sapiens 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]-pyrimidin-4(3H)-one
1.5.1.3 0.00000029
-
 pH 7.0, 37°C Homo sapiens 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.0000003
-
 pH 7.0, 37°C Homo sapiens 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.0000003
-
 pH 7.0, 37°C Homo sapiens 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.00000035
-
 pH 7.0, 37°C Homo sapiens 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.00000054
-
 pH 7.0, 37°C Homo sapiens 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.0000006
-
 pH 7.0, 37°C Homo sapiens 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.00000062
-
 pH 7.0, 37°C Homo sapiens 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.00000084
-
 pH 7.0, 37°C Homo sapiens 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000001
-
 pH 7.0, 37°C Escherichia coli N-(4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
1.5.1.3 0.0000021
-
 pH 7.0, 37°C Toxoplasma gondii N-(4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
1.5.1.3 0.0000022
-
 pH 7.0, 37°C Homo sapiens 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.0000026
-
 pH 7.0, 37°C Homo sapiens 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.0000028
-
 pH 7.0, 37°C Escherichia coli 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]-pyrimidin-4(3H)-one
1.5.1.3 0.000003
-
 pH 7.0, 37°C Escherichia coli 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.0000066
-
 pH 7.0, 37°C Homo sapiens pemetrexed
1.5.1.3 0.000008
-
 pH 7.0, 37°C Toxoplasma gondii N-(4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d ]pyrimidin-5-yl)thio]benzoyl)-L-glutamic acid
1.5.1.3 0.0000081
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.0000084
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000009
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.000012
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000013
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000014
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000014
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]-pyrimidin-4(3H)-one
1.5.1.3 0.000017
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000022
-
 pH 7.0, 37°C Escherichia coli 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000024
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000025
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000027
-
 pH 7.0, 37°C Escherichia coli 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000027
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000028
-
 pH 7.0, 37°C Escherichia coli 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000028
-
 pH 7.0, 37°C Escherichia coli 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000029
-
 pH 7.0, 37°C Escherichia coli 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000031
-
 pH 7.0, 37°C Escherichia coli 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000033
-
 pH 7.0, 37°C Toxoplasma gondii methotrexate
1.5.1.3 0.000033
-
 pH 7.0, 37°C Escherichia coli 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.000033
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.000033
-
 pH 7.0, 37°C Escherichia coli 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000033
-
 pH 7.0, 37°C Escherichia coli 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d ]pyrimidin-4(3H)-one
1.5.1.3 0.000039
-
 pH 7.0, 37°C Escherichia coli 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.000042
-
 pH 7.0, 37°C Escherichia coli 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.000087
-
 pH 7.0, 37°C Toxoplasma gondii 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
1.5.1.3 0.00023
-
 pH 7.0, 37°C Escherichia coli pemetrexed
1.5.1.3 0.00034
-
 pH 7.0, 37°C Homo sapiens trimethoprim
1.5.1.3 0.00043
-
 pH 7.0, 37°C Toxoplasma gondii pemetrexed
1.5.1.3 0.0068
-
 pH 7.0, 37°C Toxoplasma gondii trimethoprim
2.1.1.45 0.000018
-
-
 Escherichia coli N-[4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)thio]benzoyl]-L-glutamic acid
2.1.1.45 0.000019
-
-
 Escherichia coli 10-propargyl-5,8-dideazafolate
2.1.1.45 0.000036
-
-
 Toxoplasma gondii N-[4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)sulfanyl]benzoyl]-L-glutamic acid
2.1.1.45 0.00004
-
-
 Escherichia coli N-[4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)sulfanyl]benzoyl]-L-glutamic acid
2.1.1.45 0.00004
-
-
 Homo sapiens N-[4-[(2-amino-6-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)sulfanyl]benzoyl]-L-glutamic acid
2.1.1.45 0.000054
-
-
 Homo sapiens N-[4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)thio]benzoyl]-L-glutamic acid
2.1.1.45 0.000085
-
-
 Homo sapiens 10-propargyl-5,8-dideazafolate
2.1.1.45 0.00009
-
-
 Toxoplasma gondii N-[4-[(2-amino-6-ethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-5-yl)thio]benzoyl]-L-glutamic acid
2.1.1.45 0.00022
-
-
 Homo sapiens 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.00023
-
-
 Homo sapiens 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.00028
-
-
 Homo sapiens 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.00038
-
-
 Homo sapiens 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.00039
-
-
 Homo sapiens 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.00043
-
-
 Toxoplasma gondii 10-propargyl-5,8-dideazafolate
2.1.1.45 0.00069
-
-
 Homo sapiens 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]-pyrimidin-4(3H)-one
2.1.1.45 0.00075
-
-
 Homo sapiens 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.001
-
-
 Toxoplasma gondii 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.001
-
-
 Toxoplasma gondii 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0011
-
-
 Toxoplasma gondii 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0011
-
-
 Homo sapiens 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0012
-
-
 Escherichia coli 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]-pyrimidin-4(3H)-one
2.1.1.45 0.0012
-
-
 Homo sapiens 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0013
-
-
 Homo sapiens 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0014
-
-
 Escherichia coli 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0016
-
-
 Toxoplasma gondii 2-amino-5-[(3,5-dichlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]-pyrimidin-4(3H)-one
2.1.1.45 0.0017
-
-
 Escherichia coli 2-amino-6-ethyl-5-[(4-nitrophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0017
-
-
 Toxoplasma gondii 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0018
-
-
 Toxoplasma gondii 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0018
-
-
 Escherichia coli 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.002
-
-
 Escherichia coli 2-amino-6-ethyl-5-(pyridin-4-ylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.002
-
-
 Escherichia coli 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0022
-
-
 Toxoplasma gondii 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0022
-
-
 Escherichia coli 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0022
-
-
 Toxoplasma gondii 2-amino-5-[(4-bromophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0023
-
-
 Escherichia coli 2-amino-5-[(3,4-dichlorophenyl)thio]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0023
-
-
 Homo sapiens 2-amino-6-ethyl-5-(2-naphthylthio)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0023
-
-
 Escherichia coli 2-amino-5-[(3-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0023
-
-
 Toxoplasma gondii 2-amino-5-[(3,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0025
-
-
 Escherichia coli 2-amino-5-[(4-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0025
-
-
 Toxoplasma gondii 2-amino-5-[(2-chlorophenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0026
-
-
 Toxoplasma gondii 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0028
-
-
 Toxoplasma gondii pemetrexed
2.1.1.45 0.0029
-
-
 Escherichia coli 2-amino-6-ethyl-5-[(4-fluorophenyl)sulfanyl]thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0046
-
-
 Homo sapiens 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0056
-
-
 Homo sapiens 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0084
-
-
 Toxoplasma gondii 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.0095
-
-
 Homo sapiens pemetrexed
2.1.1.45 0.023
-
 IC50 above 0.023 mM Escherichia coli 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.023
-
 IC50 above 0.023 mM Toxoplasma gondii 2-amino-5-[(2,5-dimethoxyphenyl)sulfanyl]-6-ethylthieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.028
-
-
 Escherichia coli 2-amino-6-ethyl-5-(phenylsulfanyl)thieno[2,3-d]pyrimidin-4(3H)-one
2.1.1.45 0.076
-
-
 Escherichia coli pemetrexed