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Literature summary extracted from

  • Yu, Y.; Ramsay, J.A.; Ramsay, B.A.
    Production of soluble methane monooxygenase during growth of Methylosinus trichosporium on methanol (2009), J. Biotechnol., 139, 78-83.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.25 additional information when the growth-limiting substrate is switched from methane to methanol, in the presence of the particulate MMO inhibitor allylthiourea, growth continues unabated and sMMO activity is completely retained. During the brief period when both methane and methanol are present, higher sMMO activity is observed Methylosinus trichosporium OB3b

Application

EC Number Application Comment Organism
1.14.13.25 biotechnology methanol can be employed to produce large amounts of Methylosinus trichosporium biomass containing sMMO. Enzyme expression can be maintained during growth on methanol which may aid in the development of sMMO-based industrial and environmental processes Methylosinus trichosporium OB3b

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.25 Cu2+ when allylthiourea is removed, sMMO activity is maintained for an additional 24 generations, albeit at a slightly lower level due to the presence of 0.0007 mM of Cu2+ in the feed medium Methylosinus trichosporium OB3b

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.25 Methylosinus trichosporium OB3b
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.25 methane + NAD(P)H + O2
-
 Methylosinus trichosporium OB3b methanol + NAD(P)+ + H2O
-
 ?
1.14.13.25 naphthalene + NAD(P)H + O2
-
 Methylosinus trichosporium OB3b alpha-naphthol + beta-naphthol + NAD(P)+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.25 sMMO
-
 Methylosinus trichosporium OB3b
1.14.13.25 soluble methane monooxygenase
-
 Methylosinus trichosporium OB3b

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.25 NADH
-
 Methylosinus trichosporium OB3b
1.14.13.25 NADPH
-
 Methylosinus trichosporium OB3b