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Literature summary extracted from
- Purification, characterization, and gene cloning of sphingomyelinase C from Streptomyces griseocarneus NBRC13471 (2009), J. Biosci. Bioeng., 108, 293-298.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | additional information | not affected by 6% sodium taurocholate and 6% sodium deoxycholate | Streptomyces griseocarneus | |
| 3.1.4.12 | sodium cholate | 129% relative activity at 6% (v/v) sodium cholate | Streptomyces griseocarneus |  |
| 3.1.4.12 | Triton X-100 | 205% relative activity at 0.1% (v/v) Triton X-100 | Streptomyces griseocarneus | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | expressed in Escherichia coli DH5alpha cells | Streptomyces griseocarneus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | dithiothreitol | 33.1% residual activity at 2 mM | Streptomyces griseocarneus | |
| 3.1.4.12 | EDTA | complete inhibition at 2 mM | Streptomyces griseocarneus | |
| 3.1.4.12 | additional information | the enzyme is not inhibited by mercaptoethanol and iodoacetamide | Streptomyces griseocarneus | |
| 3.1.4.12 | phenylmethylsulfonyl fluoride | 70.3% residual activity at 2 mM | Streptomyces griseocarneus | |
| 3.1.4.12 | SDS | 67.7% residual activity at 2 mM | Streptomyces griseocarneus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.4.12 | Cu2+ | 107% activity at 1 mM Mg2+ | Streptomyces griseocarneus | |
| 3.1.4.12 | Fe2+ | 104% activity at 1 mM Mg2+ | Streptomyces griseocarneus | |
| 3.1.4.12 | Mg2+ | required, 100% activity at 2.5-10 mM Mg2+ | Streptomyces griseocarneus | |
| 3.1.4.12 | Mn2+ | required, 100% activity at 1 mM Mg2+ | Streptomyces griseocarneus | |
| 3.1.4.12 | additional information | not stimulated by Ca2+, CO2+, and Fe3+ | Streptomyces griseocarneus | |
| 3.1.4.12 | additional information | the order of potency to enhance the activity is Zn2+>Mn2+>Cu2+>Fe2+ | Streptomyces griseocarneus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 32102 | - |
1 * 32102, MALDI-TOF mass spectrometry | Streptomyces griseocarneus |
| 3.1.4.12 | 32130 | - |
calculated from amino acid sequence | Streptomyces griseocarneus |
| 3.1.4.12 | 38000 | - |
SDS-PAGE | Streptomyces griseocarneus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.4.12 | Streptomyces griseocarneus | A6P7M9 | - |
- |
| 3.1.4.12 | Streptomyces griseocarneus NBRC13471 | A6P7M9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.4.12 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, HiTrap Q column chromatography, Resource Q column chromatography, and Superdex 200 gel filtration | Streptomyces griseocarneus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 0.246 | - |
culture supernatant, in 50 mM glycine-NaOH buffer (pH 9.6), at 37°C | Streptomyces griseocarneus |
| 3.1.4.12 | 98.4 | - |
400fold purified enzyme, in 50 mM glycine-NaOH buffer (pH 9.0), at 55°C | Streptomyces griseocarneus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.4.12 | 1,2-di [(cis)-9-octadecenoyl]-sn-glycero-3-phospho-(1-rac-glycerol) + H2O | 2.11% activity compared to sphingomyelin | Streptomyces griseocarneus | ? | - |
? | |
| 3.1.4.12 | 1,2-di [(cis)-9-octadecenoyl]-sn-glycero-3-phospho-(1-rac-glycerol) + H2O | 2.11% activity compared to sphingomyelin | Streptomyces griseocarneus NBRC13471 | ? | - |
? | |
| 3.1.4.12 | 1,2-di(cis-9-octadecenoyl)-sn-glycerol-3-phosphate + H2O | 9.04% activity compared to sphingomyelin | Streptomyces griseocarneus | ? | - |
? | |
| 3.1.4.12 | 1,2-di(cis-9-octadecenoyl)-sn-glycerol-3-phosphate + H2O | 9.04% activity compared to sphingomyelin | Streptomyces griseocarneus NBRC13471 | ? | - |
? | |
| 3.1.4.12 | 1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O | 4.93% activity compared to sphingomyelin | Streptomyces griseocarneus | ? | - |
? | |
| 3.1.4.12 | 1,2-dioleoyl-sn-glycero-3-phospho-L-serine + H2O | 4.93% activity compared to sphingomyelin | Streptomyces griseocarneus NBRC13471 | ? | - |
? | |
| 3.1.4.12 | 1,2-dioleoyl-sn-glycero-3-phosphocholine + H2O | 0.7% activity compared to sphingomyelin | Streptomyces griseocarneus | ? | - |
? | |
| 3.1.4.12 | L-alpha-phosphatidylinositol + H2O | 5.63% activity compared to sphingomyelin | Streptomyces griseocarneus | ? | - |
? | |
| 3.1.4.12 | additional information | no activity towards 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | Streptomyces griseocarneus | ? | - |
? | |
| 3.1.4.12 | additional information | no activity towards 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine | Streptomyces griseocarneus NBRC13471 | ? | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | 100% activity | Streptomyces griseocarneus | N-acylsphingosine + choline phosphate | - |
? | |
| 3.1.4.12 | sphingomyelin + H2O | 100% activity | Streptomyces griseocarneus NBRC13471 | N-acylsphingosine + choline phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | monomer | 1 * 32102, MALDI-TOF mass spectrometry | Streptomyces griseocarneus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.4.12 | SMC | - |
Streptomyces griseocarneus |
| 3.1.4.12 | sphingomyelinase C | - |
Streptomyces griseocarneus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 50 | 55 | - |
Streptomyces griseocarneus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 30 | 60 | - |
Streptomyces griseocarneus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 4 | 60 | the enzyme activity is stable between 4°C and 37°C for 30 min, activity drops abruptly above 37°C with less than 10% activity remaining at 50-60°C | Streptomyces griseocarneus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 9 | - |
- |
Streptomyces griseocarneus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 7 | 11 | - |
Streptomyces griseocarneus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.4.12 | 5 | 10 | stable from pH 5.0-10.0, the enzyme retains activity between pH 5 and 10 for 30 min | Streptomyces griseocarneus |
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