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Literature summary extracted from

  • Ambo, T.; Noike, M.; Kurokawa, H.; Koyama, T.
    Cloning and functional analysis of cis-prenyltransferase from Thermobifida fusca (2009), J. Biosci. Bioeng., 107, 620-622.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.88
-
 Thermobifida fusca

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.88 Thermobifida fusca
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.88
-
 Thermobifida fusca

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.88 (2E,6E)-farnesyl diphosphate + n isopentenyl diphosphate about 35% of the activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + di-trans,poly-cis-polyprenyl diphosphate broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?
2.5.1.88 (2Z,6E)-farnesyl diphosphate + n isopentenyl diphosphate highest activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + trans,poly-cis-polyprenyl diphosphate n = 9-11. Broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?
2.5.1.88 geranyl diphosphate + n isopentenyl diphosphate about 5% of the activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + trans,poly-cis-polyprenyl diphosphate broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?
2.5.1.88 geranylgeranyl diphosphate + n isopentenyl diphosphate about 30% of the activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + tri-trans,poly-cis-polyprenyl diphosphate broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?
2.5.1.88 neryl diphosphate + n isopentenyl diphosphate about 10% of the activity with (2Z,6E)-farnesyl diphosphate as allylic substrate Thermobifida fusca n diphosphate + poly-cis-polyprenyl diphosphate broad product specificity with the major product being dodecaprenyl diphosphate. Synthesizes even C70 prenyl diphosphate as the maximum chain-length product ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.88 37
-
 assay at Thermobifida fusca

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.88 8
-
 assay at Thermobifida fusca