EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.46 | DHSL20 is a DHS-like gene from chromosome 20 and DHS34, a DHS gene from chromosome 34, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of His-tagged DHSL20 and DHS34 in Escherichia coli | Leishmania donovani |
2.5.1.46 | expressed in Escherichia coli as a His-tagged fusion protein | Leishmania donovani |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.46 | L344K | mutant enzyme is still devoid of any DHS activity, suggesting that other differences in amino acid sequences also contribute to its inactivity | Leishmania donovani |
2.5.1.46 | additional information | DHS34 gene deletion mutations via targeted gene replacement, chromosomal null mutants of DHS34 are only obtained in the presence of a DHS34-containing episome. HYGDELTAdhs34 and PHLEODELLTAdhs34 gene replacement cassettes are individually transfected into wild-type parasites, phenotypes, verview | Leishmania donovani |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.46 | N-guanyl-1,7-diaminoheptane | shows only little inhibitory effect on the leishmanial recombinant DHS34 | Leishmania donovani |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.46 | 0.00177 | - |
[eIF5A-precursor]-L-lysine | - |
Leishmania donovani | |
2.5.1.46 | 0.00177 | - |
[eIF5A-precursor]-lysine | pH 9.2, 37°C, recombinant DHS34 | Leishmania donovani | |
2.5.1.46 | 0.0072 | - |
spermidine | - |
Leishmania donovani | |
2.5.1.46 | 0.0232 | - |
spermidine | pH 9.2, 37°C, recombinant DHS34 | Leishmania donovani |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.46 | 42000 | - |
predicted from cDNA | Leishmania donovani |
2.5.1.46 | 48000 | - |
x * 48000, recombinant His-tagged DHSL20, SDS-PAGE | Leishmania donovani |
2.5.1.46 | 66000 | - |
predicted from cDNA | Leishmania donovani |
2.5.1.46 | 73000 | - |
2 * 66000, about, DHS34, sequence calculation, 2 * 73000, recombinant His-tagged DHS34, SDS-PAGE | Leishmania donovani |
2.5.1.46 | 137000 | - |
gel filtration | Leishmania donovani |
2.5.1.46 | 137000 | - |
recombinant DHS34, gel filtration | Leishmania donovani |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.46 | [eIF5A-precursor]-lysine + spermidine | Leishmania donovani | - |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
2.5.1.46 | [eIF5A-precursor]-lysine + spermidine | Leishmania donovani MHOM/IN/1983/AG83 | - |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.46 | Leishmania donovani | - |
two putative deoxyhypusine synthase sequences are identified in the Leishmania donovani genome | - |
2.5.1.46 | Leishmania donovani | B5APK2 | two putative deoxyhypusine synthase sequences are identified in the Leishmania donovani genome | - |
2.5.1.46 | Leishmania donovani | B5APK2 | DHS34; DHSL20 is a DHS-like gene from chromosome 20 and DHS34, a DHS gene from chromosome 34 | - |
2.5.1.46 | Leishmania donovani MHOM/IN/1983/AG83 | B5APK2 | DHS34; DHSL20 is a DHS-like gene from chromosome 20 and DHS34, a DHS gene from chromosome 34 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.46 | recombinant His-tagged DHSL20 and DHS34 from Escherichia coli by metal affinity chromatography | Leishmania donovani |
2.5.1.46 | using Ni-NTA chromatography | Leishmania donovani |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.5.1.46 | promastigote | - |
Leishmania donovani | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.46 | additional information | - |
the recombinant DHSL20 protein does not show any activity | Leishmania donovani |
2.5.1.46 | 0.0000096 | - |
low specific activity of DHS34 could be due to the fact that one of the NAD+ binding regions in the DHS34 is present in the big long insertion when compared with human deoxyhypusine synthase | Leishmania donovani |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.46 | additional information | the DHSL20 protein lacks a critical lysine residue, and the recombinant protein shows no DHS activity in vitro, while DHS34 contains the critical lysine residue, and the recombinant DHS34 effectively catalyzes deoxyhypusine synthesis | Leishmania donovani | ? | - |
? | |
2.5.1.46 | additional information | the DHSL20 protein lacks a critical lysine residue, and the recombinant protein shows no DHS activity in vitro, while DHS34 contains the critical lysine residue, and the recombinant DHS34 effectively catalyzes deoxyhypusine synthesis | Leishmania donovani MHOM/IN/1983/AG83 | ? | - |
? | |
2.5.1.46 | [eIF5A-precursor] + spermidine | - |
Leishmania donovani | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
2.5.1.46 | [eIF5A-precursor] + spermidine | - |
Leishmania donovani MHOM/IN/1983/AG83 | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
2.5.1.46 | [eIF5A-precursor]-L-lysine + spermidine | - |
Leishmania donovani | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
2.5.1.46 | [eIF5A-precursor]-L-lysine + spermidine | - |
Leishmania donovani MHOM/IN/1983/AG83 | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
2.5.1.46 | [eIF5A-precursor]-lysine + spermidine | - |
Leishmania donovani | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
2.5.1.46 | [eIF5A-precursor]-lysine + spermidine | - |
Leishmania donovani MHOM/IN/1983/AG83 | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.46 | ? | x * 48000, recombinant His-tagged DHSL20, SDS-PAGE | Leishmania donovani |
2.5.1.46 | dimer | 2 * 66000 | Leishmania donovani |
2.5.1.46 | dimer | 2 * 66000, about, DHS34, sequence calculation, 2 * 73000, recombinant His-tagged DHS34, SDS-PAGE | Leishmania donovani |
2.5.1.46 | More | structural modeling of DHS34, overview | Leishmania donovani |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.46 | deoxyhypusine synthase | - |
Leishmania donovani |
2.5.1.46 | DHS | - |
Leishmania donovani |
2.5.1.46 | DHS20 | - |
Leishmania donovani |
2.5.1.46 | DHS34 | - |
Leishmania donovani |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.46 | 37 | - |
assay at | Leishmania donovani |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.46 | 9.2 | - |
assay at | Leishmania donovani |
2.5.1.46 | 9.6 | - |
assay at | Leishmania donovani |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.46 | NAD+ | - |
Leishmania donovani | |
2.5.1.46 | NAD+ | dependent on | Leishmania donovani |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.46 | malfunction | DHSL20 lacks the catalytic lysine residue at residue 344, which is indispensable for the enzymatic reaction and is present in the active center for catalysis, and contains leucine instead | Leishmania donovani |
2.5.1.46 | malfunction | gene deletion mutations are attempted via targeted gene replacement. Chromosomal null mutants of DHS34 could only be obtained in the presence of a DHS34-containing episome, indicating that DHS34 is an essential gene | Leishmania donovani |
2.5.1.46 | additional information | structural modeling of DHS34, overview | Leishmania donovani |
2.5.1.46 | physiological function | deoxyhypusine synthase, an NAD+-dependent enzyme, catalyzes the first step in the post-translational synthesis of an unusual amino acid, hypusine (N-(4-amino-2-hydroxybutyl)lysine), in the eukaryotic initiation factor 5A precursor protein, DHS34 is essential for Leishmania donovani | Leishmania donovani |