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Literature summary extracted from

  • Lee, J.; Sperandio, V.; Frantz, D.E.; Longgood, J.; Camilli, A.; Phillips, M.A.; Michael, A.J.
    An alternative polyamine biosynthetic pathway is widespread in bacteria and essential for biofilm formation in Vibrio cholerae (2009), J. Biol. Chem., 284, 9899-9907.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.43 expressed in Escherichia coli Vibrio cholerae serotype O1
4.1.1.86 fusion protein DABA AT/DC cloned into pET28 vector, expressed in Escherichia coli BL21(DE3) Vibrio cholerae serotype O1
4.1.1.96 expressed in Escherichia coli BL21(DE3) cells Vibrio vulnificus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.86 0.17
-
L-2,4-diaminobutyrate DABA AT/DC fusion protein Vibrio cholerae serotype O1
4.1.1.86 11.1
-
L-ornithine DABA AT/DC fusion protein Vibrio cholerae serotype O1
4.1.1.96 0.1
-
carboxyspermidine pH and temperature not specified in the publication Vibrio vulnificus
4.1.1.96 0.27
-
carboxynorspermidine pH and temperature not specified in the publication Vibrio vulnificus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.43 Vibrio cholerae serotype O1
-
-
-
4.1.1.50 no activity in Vibrio cholerae
-
-
-
4.1.1.86 Vibrio cholerae serotype O1
-
El Tor strain
-
4.1.1.86 Vibrio cholerae serotype O1 El Tor
-
El Tor strain
-
4.1.1.96 Vibrio vulnificus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.86 DABA AT/DC affinity-purified Vibrio cholerae serotype O1
4.1.1.96 nitrilotriacetic acid affinity resin column chromatography Vibrio vulnificus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.43 carboxynorspermidine + H2O + NADP+
-
Vibrio cholerae serotype O1 L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
?
1.5.1.43 carboxynorspermidine + H2O + NADP+
-
Vibrio cholerae serotype O1 El Tor L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+
-
?
1.5.1.43 carboxyspermidine + H2O + NADP+
-
Vibrio cholerae serotype O1 L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
?
1.5.1.43 carboxyspermidine + H2O + NADP+
-
Vibrio cholerae serotype O1 El Tor L-aspartate 4-semialdehyde + putrescine + NADPH + H+
-
?
4.1.1.86 L-2,4-diaminobutyrate DABA AT/DC fusion protein exhibits a preference for L-2,4-diaminobutyrate over L-ornithine by 1000fold Vibrio cholerae serotype O1 ? + CO2
-
?
4.1.1.86 L-2,4-diaminobutyrate DABA AT/DC fusion protein exhibits a preference for L-2,4-diaminobutyrate over L-ornithine by 1000fold Vibrio cholerae serotype O1 El Tor ? + CO2
-
?
4.1.1.86 L-ornithine Kcat/Km is 1000fold lower than with L-2,4-diaminobutyrate Vibrio cholerae serotype O1 1,4-diaminobutane + CO2
-
?
4.1.1.86 L-ornithine Kcat/Km is 1000fold lower than with L-2,4-diaminobutyrate Vibrio cholerae serotype O1 El Tor 1,4-diaminobutane + CO2
-
?
4.1.1.96 carboxynorspermidine carboxynorspermidine is the primary substrate and is decarboxylated 20fold more efficiently than carboxyspermidine Vibrio vulnificus bis(3-aminopropyl)amine + CO2
-
?
4.1.1.96 carboxyspermidine
-
Vibrio vulnificus spermidine + CO2
-
?
4.1.1.96 L-ornithine the enzyme also exhibits some activity with L-ornithine Vibrio vulnificus ?
-
?
4.1.1.96 additional information the enzyme exhibits no activity with L-arginine, L-lysine, and meso-diaminopimelate Vibrio vulnificus ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.96 ? x * about 45000, SDS-PAGE Vibrio vulnificus

Synonyms

EC Number Synonyms Comment Organism
1.5.1.43 CANSDH
-
Vibrio cholerae serotype O1
1.5.1.43 carboxynorspermidine dehydrogenase
-
Vibrio cholerae serotype O1
1.5.1.43 VC1624
-
Vibrio cholerae serotype O1
4.1.1.86 DABA aminotransferase/decarboxylase
-
Vibrio cholerae serotype O1
4.1.1.86 DABA AT/DC
-
Vibrio cholerae serotype O1
4.1.1.86 L-2,4-diaminobutyrate aminotransferase/decarboxylase
-
Vibrio cholerae serotype O1
4.1.1.96 CANSDC
-
Vibrio vulnificus
4.1.1.96 carboxyspermidine decarboxylase
-
Vibrio vulnificus
4.1.1.96 nspC
-
Vibrio vulnificus
4.1.1.96 VC1623
-
Vibrio vulnificus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.86 0.54
-
L-ornithine DABA AT/DC fusion protein Vibrio cholerae serotype O1
4.1.1.86 8.3
-
L-2,4-diaminobutyrate DABA AT//DC fusion protein Vibrio cholerae serotype O1
4.1.1.96 3.2
-
carboxyspermidine pH and temperature not specified in the publication Vibrio vulnificus
4.1.1.96 20.1
-
carboxynorspermidine pH and temperature not specified in the publication Vibrio vulnificus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.96 pyridoxal 5'-phosphate
-
Vibrio vulnificus

General Information

EC Number General Information Comment Organism
1.5.1.43 malfunction deletion of the enzyme leads to a 50-60% reduction in growth rate of planktonic cells and severely reduced biofilm formation Vibrio cholerae serotype O1
4.1.1.86 physiological function the DABA AT/DC fusion protein is responsible for diaminopropane production Vibrio cholerae serotype O1
4.1.1.96 metabolism the CANSDH/CANSDC alternative polyamine biosynthetic pathway is able to synthesize both sym-norspermidine and spermidine Vibrio vulnificus
4.1.1.96 physiological function the enzyme is essential for biofilm formation Vibrio vulnificus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.86 0.049
-
L-ornithine DABA AT/DC fusion protein Vibrio cholerae serotype O1
4.1.1.86 49
-
L-2,4-diaminobutyrate DABA AT/DC fusion protein Vibrio cholerae serotype O1
4.1.1.96 3.2
-
carboxyspermidine pH and temperature not specified in the publication Vibrio vulnificus
4.1.1.96 74
-
carboxynorspermidine pH and temperature not specified in the publication Vibrio vulnificus