EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.43 | expressed in Escherichia coli | Vibrio cholerae serotype O1 |
4.1.1.86 | fusion protein DABA AT/DC cloned into pET28 vector, expressed in Escherichia coli BL21(DE3) | Vibrio cholerae serotype O1 |
4.1.1.96 | expressed in Escherichia coli BL21(DE3) cells | Vibrio vulnificus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.86 | 0.17 | - |
L-2,4-diaminobutyrate | DABA AT/DC fusion protein | Vibrio cholerae serotype O1 | |
4.1.1.86 | 11.1 | - |
L-ornithine | DABA AT/DC fusion protein | Vibrio cholerae serotype O1 | |
4.1.1.96 | 0.1 | - |
carboxyspermidine | pH and temperature not specified in the publication | Vibrio vulnificus | |
4.1.1.96 | 0.27 | - |
carboxynorspermidine | pH and temperature not specified in the publication | Vibrio vulnificus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.43 | Vibrio cholerae serotype O1 | - |
- |
- |
4.1.1.50 | no activity in Vibrio cholerae | - |
- |
- |
4.1.1.86 | Vibrio cholerae serotype O1 | - |
El Tor strain | - |
4.1.1.86 | Vibrio cholerae serotype O1 El Tor | - |
El Tor strain | - |
4.1.1.96 | Vibrio vulnificus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.86 | DABA AT/DC affinity-purified | Vibrio cholerae serotype O1 |
4.1.1.96 | nitrilotriacetic acid affinity resin column chromatography | Vibrio vulnificus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.43 | carboxynorspermidine + H2O + NADP+ | - |
Vibrio cholerae serotype O1 | L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+ | - |
? | |
1.5.1.43 | carboxynorspermidine + H2O + NADP+ | - |
Vibrio cholerae serotype O1 El Tor | L-aspartate 4-semialdehyde + propane-1,3-diamine + NADPH + H+ | - |
? | |
1.5.1.43 | carboxyspermidine + H2O + NADP+ | - |
Vibrio cholerae serotype O1 | L-aspartate 4-semialdehyde + putrescine + NADPH + H+ | - |
? | |
1.5.1.43 | carboxyspermidine + H2O + NADP+ | - |
Vibrio cholerae serotype O1 El Tor | L-aspartate 4-semialdehyde + putrescine + NADPH + H+ | - |
? | |
4.1.1.86 | L-2,4-diaminobutyrate | DABA AT/DC fusion protein exhibits a preference for L-2,4-diaminobutyrate over L-ornithine by 1000fold | Vibrio cholerae serotype O1 | ? + CO2 | - |
? | |
4.1.1.86 | L-2,4-diaminobutyrate | DABA AT/DC fusion protein exhibits a preference for L-2,4-diaminobutyrate over L-ornithine by 1000fold | Vibrio cholerae serotype O1 El Tor | ? + CO2 | - |
? | |
4.1.1.86 | L-ornithine | Kcat/Km is 1000fold lower than with L-2,4-diaminobutyrate | Vibrio cholerae serotype O1 | 1,4-diaminobutane + CO2 | - |
? | |
4.1.1.86 | L-ornithine | Kcat/Km is 1000fold lower than with L-2,4-diaminobutyrate | Vibrio cholerae serotype O1 El Tor | 1,4-diaminobutane + CO2 | - |
? | |
4.1.1.96 | carboxynorspermidine | carboxynorspermidine is the primary substrate and is decarboxylated 20fold more efficiently than carboxyspermidine | Vibrio vulnificus | bis(3-aminopropyl)amine + CO2 | - |
? | |
4.1.1.96 | carboxyspermidine | - |
Vibrio vulnificus | spermidine + CO2 | - |
? | |
4.1.1.96 | L-ornithine | the enzyme also exhibits some activity with L-ornithine | Vibrio vulnificus | ? | - |
? | |
4.1.1.96 | additional information | the enzyme exhibits no activity with L-arginine, L-lysine, and meso-diaminopimelate | Vibrio vulnificus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.96 | ? | x * about 45000, SDS-PAGE | Vibrio vulnificus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.43 | CANSDH | - |
Vibrio cholerae serotype O1 |
1.5.1.43 | carboxynorspermidine dehydrogenase | - |
Vibrio cholerae serotype O1 |
1.5.1.43 | VC1624 | - |
Vibrio cholerae serotype O1 |
4.1.1.86 | DABA aminotransferase/decarboxylase | - |
Vibrio cholerae serotype O1 |
4.1.1.86 | DABA AT/DC | - |
Vibrio cholerae serotype O1 |
4.1.1.86 | L-2,4-diaminobutyrate aminotransferase/decarboxylase | - |
Vibrio cholerae serotype O1 |
4.1.1.96 | CANSDC | - |
Vibrio vulnificus |
4.1.1.96 | carboxyspermidine decarboxylase | - |
Vibrio vulnificus |
4.1.1.96 | nspC | - |
Vibrio vulnificus |
4.1.1.96 | VC1623 | - |
Vibrio vulnificus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.86 | 0.54 | - |
L-ornithine | DABA AT/DC fusion protein | Vibrio cholerae serotype O1 | |
4.1.1.86 | 8.3 | - |
L-2,4-diaminobutyrate | DABA AT//DC fusion protein | Vibrio cholerae serotype O1 | |
4.1.1.96 | 3.2 | - |
carboxyspermidine | pH and temperature not specified in the publication | Vibrio vulnificus | |
4.1.1.96 | 20.1 | - |
carboxynorspermidine | pH and temperature not specified in the publication | Vibrio vulnificus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.96 | pyridoxal 5'-phosphate | - |
Vibrio vulnificus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.5.1.43 | malfunction | deletion of the enzyme leads to a 50-60% reduction in growth rate of planktonic cells and severely reduced biofilm formation | Vibrio cholerae serotype O1 |
4.1.1.86 | physiological function | the DABA AT/DC fusion protein is responsible for diaminopropane production | Vibrio cholerae serotype O1 |
4.1.1.96 | metabolism | the CANSDH/CANSDC alternative polyamine biosynthetic pathway is able to synthesize both sym-norspermidine and spermidine | Vibrio vulnificus |
4.1.1.96 | physiological function | the enzyme is essential for biofilm formation | Vibrio vulnificus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.86 | 0.049 | - |
L-ornithine | DABA AT/DC fusion protein | Vibrio cholerae serotype O1 | |
4.1.1.86 | 49 | - |
L-2,4-diaminobutyrate | DABA AT/DC fusion protein | Vibrio cholerae serotype O1 | |
4.1.1.96 | 3.2 | - |
carboxyspermidine | pH and temperature not specified in the publication | Vibrio vulnificus | |
4.1.1.96 | 74 | - |
carboxynorspermidine | pH and temperature not specified in the publication | Vibrio vulnificus |