EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.32 | complementation of the KN99alpha xpt1DELTA strain by episomal expression of XPT1 under the control of its native promoter (pXPT1) restores the manganese-dependent xylosyltransferase activity to wild-type levels | Cryptococcus neoformans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.32 | EDTA | - |
Cryptococcus neoformans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.8.32 | membrane | the Xpt1 sequence contains a single preducted transmembrane domain | Cryptococcus neoformans | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.8.32 | Co2+ | 15% of the activity compared to Mn2+ as metal ion cofactor | Cryptococcus neoformans | |
2.7.8.32 | Mg2+ | 15% of the activity compared to Mn2+ as metal ion cofactor | Cryptococcus neoformans | |
2.7.8.32 | Mn2+ | preferred metal ion cofactor | Cryptococcus neoformans | |
2.7.8.32 | additional information | no activity in presence of Ca2+, Cu2+, Ni2+, Zn2+ or Fe2+ | Cryptococcus neoformans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.8.32 | 99527 | - |
x * 99527, calculated from sequence | Cryptococcus neoformans |
2.7.8.32 | 99530 | - |
calculated from sequence | Cryptococcus neoformans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.32 | Cryptococcus neoformans | D0U690 | var. grubii | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.32 | affinity purification | Cryptococcus neoformans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.32 | additional information | the enzyme is specific for Man as an acceptor but is flexible as to the structural context of the mannosyl disaccharide | Cryptococcus neoformans | ? | - |
? | |
2.7.8.32 | UDP-xylose + 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose | high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor | Cryptococcus neoformans | UMP + 2-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose | - |
? | |
2.7.8.32 | UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose | high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor | Cryptococcus neoformans | UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose | - |
? | |
2.7.8.32 | UDP-xylose + 4-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose | high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor | Cryptococcus neoformans | UMP + 4-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose | - |
? | |
2.7.8.32 | UDP-xylose + 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose | high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor | Cryptococcus neoformans | UMP + 6-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.8.32 | ? | x * 99527, calculated from sequence | Cryptococcus neoformans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.32 | XPT1 | - |
Cryptococcus neoformans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.8.32 | 20 | - |
assay at | Cryptococcus neoformans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.8.32 | 6.5 | - |
assay at | Cryptococcus neoformans |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.7.8.32 | malfunction | deletion of XPT1 yields a corresponding loss of the manganese-dependent xylosyltransferase product | Cryptococcus neoformans |