BRENDA - Enzyme Database

A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

Reilly, M.C.; Levery, S.B.; Castle, S.A.; Klutts, J.S.; Doering, T.L.; J. Biol. Chem. 284, 36118-36127 (2009) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
2.7.8.32
complementation of the KN99alpha xpt1DELTA strain by episomal expression of XPT1 under the control of its native promoter (pXPT1) restores the manganese-dependent xylosyltransferase activity to wild-type levels
Cryptococcus neoformans
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.32
EDTA
-
Cryptococcus neoformans
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.32
membrane
the Xpt1 sequence contains a single preducted transmembrane domain
Cryptococcus neoformans
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.32
Co2+
15% of the activity compared to Mn2+ as metal ion cofactor
Cryptococcus neoformans
2.7.8.32
Mg2+
15% of the activity compared to Mn2+ as metal ion cofactor
Cryptococcus neoformans
2.7.8.32
Mn2+
preferred metal ion cofactor
Cryptococcus neoformans
2.7.8.32
additional information
no activity in presence of Ca2+, Cu2+, Ni2+, Zn2+ or Fe2+
Cryptococcus neoformans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.32
99527
-
x * 99527, calculated from sequence
Cryptococcus neoformans
2.7.8.32
99530
-
calculated from sequence
Cryptococcus neoformans
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.8.32
Cryptococcus neoformans
D0U690
var. grubii
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.7.8.32
affinity purification
Cryptococcus neoformans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2.7.8.32
additional information
the enzyme is specific for Man as an acceptor but is flexible as to the structural context of the mannosyl disaccharide
704602
Cryptococcus neoformans
?
-
-
-
?
2.7.8.32
UDP-xylose + 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 2-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
2.7.8.32
UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
2.7.8.32
UDP-xylose + 4-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 4-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
2.7.8.32
UDP-xylose + 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 6-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.32
?
x * 99527, calculated from sequence
Cryptococcus neoformans
Synonyms
EC Number
Synonyms
Commentary
Organism
2.7.8.32
XPT1
-
Cryptococcus neoformans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.32
20
-
assay at
Cryptococcus neoformans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.32
6.5
-
assay at
Cryptococcus neoformans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.32
complementation of the KN99alpha xpt1DELTA strain by episomal expression of XPT1 under the control of its native promoter (pXPT1) restores the manganese-dependent xylosyltransferase activity to wild-type levels
Cryptococcus neoformans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.32
EDTA
-
Cryptococcus neoformans
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.32
membrane
the Xpt1 sequence contains a single preducted transmembrane domain
Cryptococcus neoformans
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.32
Co2+
15% of the activity compared to Mn2+ as metal ion cofactor
Cryptococcus neoformans
2.7.8.32
Mg2+
15% of the activity compared to Mn2+ as metal ion cofactor
Cryptococcus neoformans
2.7.8.32
Mn2+
preferred metal ion cofactor
Cryptococcus neoformans
2.7.8.32
additional information
no activity in presence of Ca2+, Cu2+, Ni2+, Zn2+ or Fe2+
Cryptococcus neoformans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.32
99527
-
x * 99527, calculated from sequence
Cryptococcus neoformans
2.7.8.32
99530
-
calculated from sequence
Cryptococcus neoformans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.32
affinity purification
Cryptococcus neoformans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2.7.8.32
additional information
the enzyme is specific for Man as an acceptor but is flexible as to the structural context of the mannosyl disaccharide
704602
Cryptococcus neoformans
?
-
-
-
?
2.7.8.32
UDP-xylose + 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 2-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
2.7.8.32
UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
2.7.8.32
UDP-xylose + 4-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 4-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
2.7.8.32
UDP-xylose + 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 6-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.32
?
x * 99527, calculated from sequence
Cryptococcus neoformans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.32
20
-
assay at
Cryptococcus neoformans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.32
6.5
-
assay at
Cryptococcus neoformans
General Information
EC Number
General Information
Commentary
Organism
2.7.8.32
malfunction
deletion of XPT1 yields a corresponding loss of the manganese-dependent xylosyltransferase product
Cryptococcus neoformans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.7.8.32
malfunction
deletion of XPT1 yields a corresponding loss of the manganese-dependent xylosyltransferase product
Cryptococcus neoformans