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Literature summary extracted from

  • Reilly, M.C.; Levery, S.B.; Castle, S.A.; Klutts, J.S.; Doering, T.L.
    A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans (2009), J. Biol. Chem., 284, 36118-36127.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.32 complementation of the KN99alpha xpt1DELTA strain by episomal expression of XPT1 under the control of its native promoter (pXPT1) restores the manganese-dependent xylosyltransferase activity to wild-type levels Cryptococcus neoformans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.32 EDTA
-
Cryptococcus neoformans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.32 membrane the Xpt1 sequence contains a single preducted transmembrane domain Cryptococcus neoformans 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.32 Co2+ 15% of the activity compared to Mn2+ as metal ion cofactor Cryptococcus neoformans
2.7.8.32 Mg2+ 15% of the activity compared to Mn2+ as metal ion cofactor Cryptococcus neoformans
2.7.8.32 Mn2+ preferred metal ion cofactor Cryptococcus neoformans
2.7.8.32 additional information no activity in presence of Ca2+, Cu2+, Ni2+, Zn2+ or Fe2+ Cryptococcus neoformans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.32 99527
-
x * 99527, calculated from sequence Cryptococcus neoformans
2.7.8.32 99530
-
calculated from sequence Cryptococcus neoformans

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.32 Cryptococcus neoformans D0U690 var. grubii
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.32 affinity purification Cryptococcus neoformans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.32 additional information the enzyme is specific for Man as an acceptor but is flexible as to the structural context of the mannosyl disaccharide Cryptococcus neoformans ?
-
?
2.7.8.32 UDP-xylose + 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor Cryptococcus neoformans UMP + 2-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
?
2.7.8.32 UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor Cryptococcus neoformans UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
?
2.7.8.32 UDP-xylose + 4-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor Cryptococcus neoformans UMP + 4-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
?
2.7.8.32 UDP-xylose + 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor Cryptococcus neoformans UMP + 6-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
?

Subunits

EC Number Subunits Comment Organism
2.7.8.32 ? x * 99527, calculated from sequence Cryptococcus neoformans

Synonyms

EC Number Synonyms Comment Organism
2.7.8.32 XPT1
-
Cryptococcus neoformans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.32 20
-
assay at Cryptococcus neoformans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.32 6.5
-
assay at Cryptococcus neoformans

General Information

EC Number General Information Comment Organism
2.7.8.32 malfunction deletion of XPT1 yields a corresponding loss of the manganese-dependent xylosyltransferase product Cryptococcus neoformans