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Literature summary extracted from

  • Jimenez, N.; Vilches, S.; Lacasta, A.; Regué, M.; Merino, S.; Tomás, J.
    A bifunctional enzyme in a single gene catalyzes the incorporation of GlcN into the Aeromonas core lipopolysaccharide (2009), J. Biol. Chem., 284, 32995-33005.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.56 overexpression in Escherichia coli BL21 Aeromonas hydrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.56 0.016
-
 lipopolysaccharide
-
 Aeromonas hydrophila
2.4.1.56 0.04
-
 UDP-N-acetyl-D-glucosamine
-
 Aeromonas hydrophila
2.4.1.56 0.052
-
 lipopolysaccharide mutants with recombinant plasmids expression of C- or N-domains Aeromonas hydrophila
2.4.1.56 0.119
-
 UDP-N-acetyl-D-glucosamine mutants with recombinant plasmids expression of C- or N-domains Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.56 Aeromonas hydrophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.56 His-Tag affinity chromatography Aeromonas hydrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.56 UDP-N-acetyl-D-glucosamine + lipopolysaccharide
-
 Aeromonas hydrophila ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.56 bifunctional glycosyltransferase
-
 Aeromonas hydrophila
2.4.1.56 WahA glycosyltransferase
-
 Aeromonas hydrophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.56 1
-
 UDP-N-acetyl-D-glucosamine mutants with recombinant plasmids expression of C- or N-domains Aeromonas hydrophila
2.4.1.56 2.5
-
 lipopolysaccharide mutants with recombinant plasmids expression of C- or N-domains Aeromonas hydrophila
2.4.1.56 10
-
 UDP-N-acetyl-D-glucosamine
-
 Aeromonas hydrophila
2.4.1.56 25
-
 lipopolysaccharide
-
 Aeromonas hydrophila