EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.3 | ligated into pET21-b vector, expressed from pZCH2 in Escherichia coli BL21 (lambdaDE3) cell line | Vibrio harveyi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.14.3 | D262A | 90% reduced activity with octanal, 36% reduced activity with decanal, activity with dodecanal as the wild-type | Vibrio harveyi |
1.14.14.3 | D265A | activity with octanal as the wild-type, 81% reduced activity with decanal, complete loss of dodecanal activity | Vibrio harveyi |
1.14.14.3 | D271A | complete loss of octanal and decanal activity, 18% reduced activity with dodecanal | Vibrio harveyi |
1.14.14.3 | H285A | 26% reduced activity with octanal, 74% reduced activity with decanal, complete loss of dodecanal activity | Vibrio harveyi |
1.14.14.3 | K274A | 89% reduced activity with octanal, 21% reduced activity with decanal, 81% reduced activity with dodecanal | Vibrio harveyi |
1.14.14.3 | K283A | complete loss of octanal and decanal activity, 96% reduced activity with dodecanal, does not significantly impede binding of decanal, results in destabilization of intermediate II, results in a loss in quantum yield comparable with that of the loop deletion mutant, binds reduced flavin more weakly | Vibrio harveyi |
1.14.14.3 | K286A | 92% reduced activity with octanal, complete loss of decanal activity, 87% reduced activity with dodecanal, does not significantly impede binding of decanal, increase in exposure of reaction intermediates to a dynamic quencher, results in a loss in quantum yield comparable with that of the loop deletion mutant, binds reduced flavin more weakly | Vibrio harveyi |
1.14.14.3 | R291A | 77% reduced activity with octanal, 58% reduced activity with decanal, 71% reduced activity with dodecanal | Vibrio harveyi |
1.14.14.3 | W277A | 11% reduced activity with octanal, 50% reduced activity with decanal and dodecanal | Vibrio harveyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.3 | 0.008 | - |
decanal | wild-type | Vibrio harveyi | |
1.14.14.3 | 0.01 | - |
decanal | mutant K286A | Vibrio harveyi | |
1.14.14.3 | 0.0105 | - |
decanal | mutant K283A | Vibrio harveyi |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.3 | Vibrio harveyi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.14.3 | on nickel affinity column, to more than 90% purity | Vibrio harveyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.3 | decanal + FMNH + O2 | - |
Vibrio harveyi | decanoic acid + FMN + H2O + light | - |
? | |
1.14.14.3 | dodecanal + FMNH + O2 | - |
Vibrio harveyi | dodecanoic acid + FMN + H2O + light | - |
? | |
1.14.14.3 | octanal + FMNH + O2 | - |
Vibrio harveyi | octanoic acid + FMN + H2O + light | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.3 | bacterial luciferase | - |
Vibrio harveyi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.3 | FMN | - |
Vibrio harveyi |