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Literature summary extracted from

  • Morii, H.; Kiyonari, S.; Ishino, Y.; Koga, Y.
    A novel biosynthetic pathway of archaetidyl-myo-inositol via archaetidyl-myo-inositol phosphate from CDP-archaeol and D-glucose 6-phosphate in methanoarchaeon Methanothermobacter thermautotrophicus cells (2009), J. Biol. Chem., 284, 30766-30774.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.39 Triton X-100 0.5%, stimulates Methanothermobacter thermautotrophicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.39 expression in Escherichia coli Methanothermobacter thermautotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.B14 phosphate 8–20 mM phosphate buffer inhibits Methanothermobacter thermautotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.39 membrane
-
 Pyrococcus furiosus 16020
-
2.7.8.39 membrane
-
 Methanothermobacter thermautotrophicus 16020
-
3.1.3.B14 membrane
-
 Methanothermobacter thermautotrophicus 16020
-
5.5.1.4 membrane
-
 Methanothermobacter thermautotrophicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.39 Mg2+ Mg2+ or Mn2+ required Methanothermobacter thermautotrophicus
2.7.8.39 Mn2+ Mg2+ or Mn2+ required Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.39 20764
-
 x * 20764, calculated from sequence Methanothermobacter thermautotrophicus
2.7.8.39 20900
-
 x * 20900, SDS-PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.39 CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate Methanothermobacter thermautotrophicus the enzyme is involved in biosynthesis of archaetidyl-myo-inositol CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
 ?
3.1.3.B14 1-archaetidyl-1D-myo-inositol 3-phosphate + H2O Methanothermobacter thermautotrophicus the enzyme is involved in biosynthesis of archaetidyl-myo-inositol, a compound essential for glycolipid biosynthesis in archaea 1-archaetidyl-1D-myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.39 Methanothermobacter thermautotrophicus O27726
-
-
2.7.8.39 Pyrococcus furiosus
-
-
-
3.1.3.B14 Methanothermobacter thermautotrophicus
-
-
-
5.5.1.4 Methanothermobacter thermautotrophicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.4 partially purified by ammonium sulfate fractionation (50-70%) Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.39 0.02
-
 pH 8.0, 60°C, crude enzyme Methanothermobacter thermautotrophicus
5.5.1.4 0.0003
-
 crude enzyme, in 50mM Bicine buffer (pH 8.5), at 60°C Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.39 CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
 Methanothermobacter thermautotrophicus CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
 ?
2.7.8.39 CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate the enzyme is involved in biosynthesis of archaetidyl-myo-inositol Methanothermobacter thermautotrophicus CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
 ?
3.1.3.B14 1-archaetidyl-1D-myo-inositol 3-phosphate + H2O
-
 Methanothermobacter thermautotrophicus 1-archaetidyl-1D-myo-inositol + phosphate
-
 ?
3.1.3.B14 1-archaetidyl-1D-myo-inositol 3-phosphate + H2O the enzyme is involved in biosynthesis of archaetidyl-myo-inositol, a compound essential for glycolipid biosynthesis in archaea Methanothermobacter thermautotrophicus 1-archaetidyl-1D-myo-inositol + phosphate
-
 ?
5.5.1.4 D-glucose 6-phosphate
-
 Methanothermobacter thermautotrophicus 1L-myo-inositol 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.39 ? x * 20900, SDS-PAGE Methanothermobacter thermautotrophicus
2.7.8.39 ? x * 20764, calculated from sequence Methanothermobacter thermautotrophicus

Synonyms

EC Number Synonyms Comment Organism
2.7.8.39 AIP synthase
-
 Methanothermobacter thermautotrophicus
2.7.8.39 MTH1691
-
 Methanothermobacter thermautotrophicus
3.1.3.B14 AIP phosphatase
-
 Methanothermobacter thermautotrophicus
5.5.1.4 Inositol 1-phosphate synthase
-
 Methanothermobacter thermautotrophicus
5.5.1.4 IP synthase
-
 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.39 60
-
 assay at Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.39 8
-
 assay at Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
5.5.1.4 NAD+
-
 Methanothermobacter thermautotrophicus