EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.44 | His-tag, expressed in Escherichia coli BL21(de3)plysS | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.44 | A386S | possibly critical role in catalysis, relative activity: 90% (wild-type: 100%) | Arabidopsis thaliana |
2.7.1.44 | A41E | possibly critical for sugar binding, relative activity: 0% (wild-type: 100%) | Arabidopsis thaliana |
2.7.1.44 | Y250F | possibly important for substrate specificity (galacturonic acid /glucuronic acid), relative activity: 90% (wild-type: 100%), 50% activity with D-glucuronic acid (wild-type: 0%) | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.44 | ADP | addition (4 mM) results in 40% reduction of activity | Arabidopsis thaliana | |
2.7.1.44 | EDTA | - |
Arabidopsis thaliana | |
2.7.1.44 | additional information | no inhibition is observed by CTP, GTP ITP,TTP, or UTP (4 mM) | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.44 | 0.0708 | - |
D-galacturonate | wild-type protein | Arabidopsis thaliana | |
2.7.1.44 | 0.195 | - |
ATP | wild-type protein | Arabidopsis thaliana | |
2.7.1.44 | 0.395 | - |
D-galacturonate | Y250F mutant protein | Arabidopsis thaliana |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.44 | Mg2+ | Mn2+ (32% relative activity) and Ca2+ (61% relative activity) can substitute for Mg2+ | Arabidopsis thaliana |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.44 | Arabidopsis thaliana | Q8VYG2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.44 | - |
Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.44 | flower | - |
Arabidopsis thaliana | - |
2.7.1.44 | leaf | - |
Arabidopsis thaliana | - |
2.7.1.44 | root | - |
Arabidopsis thaliana | - |
2.7.1.44 | silique | - |
Arabidopsis thaliana | - |
2.7.1.44 | stem | - |
Arabidopsis thaliana | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.44 | -20°C, crude recombinant enzyme is stable | Arabidopsis thaliana |
2.7.1.44 | -20°C, purified enzyme preparations lose more than 90% of activity within one week | Arabidopsis thaliana |
2.7.1.44 | -80°C, stable for several months when diluted with glycerol to 25% (v/v) and flash-frozen in liquid nitrogen | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.44 | D-galacturonate + ATP | wild-type protein: no activity with D-galactose, D-glucose, D-fructose, D-mannose, D-gluconic acid, D-xylose, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, D-glucosamine, D-arabinose, L-arabinose, L-rhamnose,or L-Fucose, these monosaccharides (4 mM) have no effect on activity in the presence of galacturonic acid, neither CTP, GTP ITP,TTP, nor UTP are substrates | Arabidopsis thaliana | 1-phospho-alpha-D-galacturonate + ADP | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.44 | alpha-D-galacturonic acid kinase | - |
Arabidopsis thaliana |
2.7.1.44 | alpha-D-galacturonic acid-1-phosphate kinase | - |
Arabidopsis thaliana |
2.7.1.44 | GalAK | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.44 | 37 | - |
- |
Arabidopsis thaliana |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.44 | 4 | 42 | - |
Arabidopsis thaliana |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.44 | 55 | - |
no activity at 55°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.44 | 7.5 | 7.8 | - |
Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.44 | 3.3 | 9.5 | - |
Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.44 | 0.76 | - |
Alpha-D-glucuronic acid | Y250F mutant protein | Arabidopsis thaliana | |
2.7.1.44 | 6.3 | - |
ATP | wild-type protein | Arabidopsis thaliana | |
2.7.1.44 | 24 | - |
D-galacturonate | Y250F mutant protein | Arabidopsis thaliana | |
2.7.1.44 | 36 | - |
D-galacturonate | wild-type protein | Arabidopsis thaliana |