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Literature summary extracted from

  • Saggu, M.; Zebger, I.; Ludwig, M.; Lenz, O.; Friedrich, B.; Hildebrandt, P.; Lendzian, F.
    Spectroscopic insights into the oxygen-tolerant membrane-associated [NiFe] hydrogenase of Ralstonia eutropha H16 (2009), J. Biol. Chem., 284, 16264-16276.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.99.6 overexpressed in a non-tagged version using plasmid pLO6, harboring the complete MBH operon, in Ralstonia eutropha strain HF631 Cupriavidus necator H16

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.99.6 additional information is oxygen-tolerant Cupriavidus necator H16

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.12.99.6 membrane the hydrogenase dimer is electronically connected to a membrane-integral cytochrome b Cupriavidus necator H16 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.99.6 CN- the catalytic center is equipped with 1.8 CN- per protein molecule Cupriavidus necator H16
1.12.99.6 CO the catalytic center is equipped with one CO Cupriavidus necator H16
1.12.99.6 Fe is composed of a small subunit, capable in coordinating one [3Fe4S] and two [4Fe4S] clusters Cupriavidus necator H16
1.12.99.6 Ni2+ is composed of a large subunit, harboring the [NiFe] active site Cupriavidus necator H16

Organism

EC Number Organism UniProt Comment Textmining
1.12.99.6 Cupriavidus necator H16
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.99.6 by affinity chromatography, ca. 99% pure Cupriavidus necator H16

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.99.6 H2 + oxidized acceptor almost 100% of the membrane-attached MBH is reversibly redox-active Cupriavidus necator H16 H+ + reduced acceptor
-
 ?

Subunits

EC Number Subunits Comment Organism
1.12.99.6 dimer detachment of the enzyme from the membrane Cupriavidus necator H16
1.12.99.6 trimer in its native environment Cupriavidus necator H16

Synonyms

EC Number Synonyms Comment Organism
1.12.99.6 Mbh
-
 Cupriavidus necator H16
1.12.99.6 [NiFe] hydrogenase
-
 Cupriavidus necator H16

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.12.99.6 additional information
-
 solubilization of the imer is associated with a downshift of the optimum pH for H2-oxidizing activity Cupriavidus necator H16