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Literature summary extracted from

  • Kumaran, S.; Yi, H.; Krishnan, H.B.; Jez, J.M.
    Assembly of the cysteine synthase complex and the regulatory role of protein-protein interactions (2009), J. Biol. Chem., 284, 10268-10275.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.47 expressed in Escherichia coli as a His-tagged fusion protein Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.47 67600
-
 calculated from cDNA Glycine max
2.5.1.47 70000
-
 gel filtration Glycine max
2.5.1.47 74200
-
 sedimentation equilibrium ultracentrifugation Glycine max

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.47 Glycine max Q8W1A0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.47 purified using nickel-affinity and gel filtration. Thrombin digestion is used to remove the His tag from each protein Glycine max

Subunits

EC Number Subunits Comment Organism
2.5.1.47 homodimer gel filtration Glycine max
2.5.1.47 More a cysteine synthase complex is formed by association of serine O-acetyltransferase (SAT) and O-acetylserine sulfhydrylase (OASS). Biophysical examination cysteine synthase complex by gel filtration and sedimentation ultracentrifugation indicates that this assembly consists of a single serine O-trimer and three OASS dimers Glycine max

Synonyms

EC Number Synonyms Comment Organism
2.5.1.47 O-acetylserine sulfhydrylase
-
 Glycine max
2.5.1.47 OASS
-
 Glycine max