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Literature summary extracted from

  • Kamijo, S.; Fujii, A.; Onodera, K.; Wakabayashi, K.
    Analyses of conditions for KMSSS loop in tyrosyl-tRNA synthetase by building a mutant library (2009), J. Biochem., 146, 241-250.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.1 a library of more than 200 mutants substituting the ATP binding motif KMSSS, Lys204-Met205-Ser206-Ser207-Ser208, is built, mutants and wild-type of MjYRS are cloned into the vector pET41a+ Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.1 AMSSS TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built Methanocaldococcus jannaschii
6.1.1.1 KMGCA TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built Methanocaldococcus jannaschii
6.1.1.1 RMSSS TyrRS mutant, a library of more than 200 mutants substituting the ATP binding motif KMSSS is built Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.1 0.0015
-
 L-tyrosine mutant TyrRS, KMGCA Methanocaldococcus jannaschii
6.1.1.1 0.0043
-
 L-tyrosine mutant TyrRS, RMSSS Methanocaldococcus jannaschii
6.1.1.1 0.0043
-
 L-tyrosine wild-type TyrRS, KMSSS Methanocaldococcus jannaschii
6.1.1.1 0.0044
-
 L-tyrosine mutant TyrRS, AMSSS Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii
-
 AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.1 Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.1 by chromatography on Ni-nitrilotriacetic acid agarose Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.1 ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.1 MjYRS
-
 Methanocaldococcus jannaschii
6.1.1.1 Tyrosyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
6.1.1.1 TyrRS
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.1 37
-
 tyrosylation assay Methanocaldococcus jannaschii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.1 0.01
-
 L-tyrosine mutant TyrRS, RMSSS Methanocaldococcus jannaschii
6.1.1.1 0.012
-
 L-tyrosine mutant TyrRS, AMSSS Methanocaldococcus jannaschii
6.1.1.1 0.025
-
 L-tyrosine mutant TyrRS, KMGCA Methanocaldococcus jannaschii
6.1.1.1 0.53
-
 L-tyrosine wild-type TyrRS, KMSSS Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.1 7.5
-
 tyrosylation assay Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.1 ATP
-
 Methanocaldococcus jannaschii