Literature summary extracted from

Nemoto, N.; Oshima, T.; Yamagishi, A.
Purification and characterization of geranylgeranylglyceryl phosphate synthase from a thermoacidophilic archaeon, Thermoplasma acidophilum (2003), J. Biochem., 133, 651-657.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
2.5.1.41	Triton X-100	required for enzymatic activity, optimal concentration: 0.01%	Thermoplasma acidophilum

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.5.1.41	recombinantly expressed in Escherichia coli	Thermoplasma acidophilum

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.5.1.41	EDTA	-	Thermoplasma acidophilum

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.5.1.41	0.000075	-	geranylgeranyl diphosphate	-	Thermoplasma acidophilum
2.5.1.41	0.0212	-	sn-glycerol 1-phosphate	-	Thermoplasma acidophilum

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.5.1.41	Mg2+	optimal concentration 5-10 mM	Thermoplasma acidophilum

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.5.1.41	27000	-	SDS-PAGE	Thermoplasma acidophilum
2.5.1.41	50000	-	gel filtration	Thermoplasma acidophilum

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.5.1.41	Thermoplasma acidophilum	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.5.1.41	using DEAE-cellulose column chromatography, NH4SO4 precipitation, Phenyl-650S and Resourse Q columns, Mini Q column and Superdex 200 HR 10/30 column	Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.5.1.41	geranylgeranyl diphosphate + sn-glycerol 1-phosphate	-	Thermoplasma acidophilum	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
2.5.1.41	homodimer	gel filtration, 2 * 27000	Thermoplasma acidophilum

Synonyms

EC Number	Synonyms	Comment	Organism
2.5.1.41	geranylgeranylglyceryl phosphate synthase	-	Thermoplasma acidophilum
2.5.1.41	GGGP synthase	-	Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
2.5.1.41	55	-	assay at and optimal temperature	Thermoplasma acidophilum

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.5.1.41	7	-	assay at and optimal pH	Thermoplasma acidophilum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)