EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.20 | expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells | Picrophilus torridus |
4.1.2.51 | expression in Escherichia coli | Picrophilus torridus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.20 | 0.3 | - |
2-dehydro-3-deoxy-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
4.1.2.20 | 8 | - |
2-dehydro-3-deoxy-6-phospho-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
4.1.2.51 | 0.3 | - |
2-dehydro-3-deoxy-D-gluconate | pH 6.2, 60°C, enzyme purified from Picrophilus torridus | Picrophilus torridus | |
4.1.2.51 | 0.4 | - |
2-dehydro-3-deoxy-D-gluconate | pH 6.2, 60°C, recombinant enzyme | Picrophilus torridus | |
4.1.2.51 | 2.5 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | pH 6.2, 60°C, recombinant enzyme | Picrophilus torridus | |
4.1.2.51 | 2.7 | - |
pyruvate | pH 6.2, 60°C | Picrophilus torridus | |
4.1.2.51 | 4.6 | - |
D-glyceraldehyde | pH 6.2, 60°C | Picrophilus torridus | |
4.1.2.51 | 8 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | pH 6.2, 60°C, enzyme purified from Picrophilus torridus | Picrophilus torridus | |
4.2.1.39 | 2 | - |
D-galactonate | - |
Picrophilus torridus | |
4.2.1.39 | 2.5 | - |
gluconate | - |
Picrophilus torridus | |
4.2.1.140 | 2 | - |
D-galactonate | pH 6.2, 60°C | Picrophilus torridus | |
4.2.1.140 | 2.5 | - |
D-gluconate | pH 6.2, 60°C | Picrophilus torridus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.39 | Mg2+ | is required | Picrophilus torridus | |
4.2.1.140 | Mg2+ | required, Km: 4.2 mM | Picrophilus torridus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.20 | 32000 | - |
4 * 32000, SDS-PAGE | Picrophilus torridus |
4.1.2.20 | 120000 | - |
gel filtration | Picrophilus torridus |
4.1.2.51 | 32000 | - |
4 * 32000, SDS-PAGE | Picrophilus torridus |
4.1.2.51 | 120000 | - |
gel filtration | Picrophilus torridus |
4.2.1.39 | 44000 | - |
8 * 44000, SDS-PAGE | Picrophilus torridus |
4.2.1.39 | 340000 | - |
gel filtration | Picrophilus torridus |
4.2.1.140 | 44000 | - |
8 * 44000, SDS-PAGE | Picrophilus torridus |
4.2.1.140 | 340000 | - |
gel filtration | Picrophilus torridus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.51 | 2-dehydro-3-deoxy-D-gluconate | Picrophilus torridus | the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway | pyruvate + D-glyceraldehyde | - |
? | |
4.2.1.140 | D-galactonate | Picrophilus torridus | - |
2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
4.2.1.140 | D-gluconate | Picrophilus torridus | - |
2-dehydro-3-deoxy-D-gluconate + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.20 | Picrophilus torridus | - |
- |
- |
4.1.2.51 | Picrophilus torridus | Q6KZI8 | - |
- |
4.2.1.39 | Picrophilus torridus | Q6L1T2 | - |
- |
4.2.1.140 | Picrophilus torridus | Q6L1T2 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.20 | ammonium sulfate precipitation, phenyl-Sepharose column chromatography, UnoQ5 column chromatography, and Superdex 200 gel filtration | Picrophilus torridus |
4.1.2.51 | - |
Picrophilus torridus |
4.2.1.39 | by centrifugation and gel filtration, 190fold to apparent homogeneity | Picrophilus torridus |
4.2.1.140 | - |
Picrophilus torridus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.2.20 | culture condition:glucose-grown cell | - |
Picrophilus torridus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.2.20 | 0.14 | - |
crude extract, at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus |
4.1.2.20 | 48 | - |
after 343fold purification, at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus |
4.1.2.51 | 48 | - |
pH 6.2, 60°C, substrate: 2-dehydro-3-deoxy-D-gluconate | Picrophilus torridus |
4.2.1.140 | 5.1 | - |
substrate: D-gluconate, pH 6.2, 60°C | Picrophilus torridus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.20 | 2-dehydro-3-deoxy-6-phospho-D-gluconate | - |
Picrophilus torridus | pyruvate + glyceraldehyde-3-phosphate | - |
? | |
4.1.2.20 | 2-dehydro-3-deoxy-D-gluconate | the enzyme is highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate | Picrophilus torridus | pyruvate + glyceraldehyde | - |
? | |
4.1.2.51 | 2-dehydro-3-deoxy-D-galactonate | - |
Picrophilus torridus | pyruvate + D-glyceraldehyde | - |
? | |
4.1.2.51 | 2-dehydro-3-deoxy-D-galactonate | the enzyme shows substrate promiscuity, as measured in the direction of aldol synthesis. The enzyme catalyzes the formation of both 2-dehydro-3-deoxy-D-gluconate and its C-4 epimer 2-keto-3-deoxygalactonate from D- or L-glyceraldehyde and pyruvate at a similar ratio, indicating lack of stereospecific control in formation and cleavage of 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate | Picrophilus torridus | pyruvate + D-glyceraldehyde | - |
r | |
4.1.2.51 | 2-dehydro-3-deoxy-D-gluconate | the enzyme from the archaeon Picrophilus torridus is involved in D-glucose and D-galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway | Picrophilus torridus | pyruvate + D-glyceraldehyde | - |
? | |
4.1.2.51 | 2-dehydro-3-deoxy-D-gluconate | highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate | Picrophilus torridus | pyruvate + D-glyceraldehyde | - |
? | |
4.1.2.51 | 2-dehydro-3-deoxy-D-gluconate 6-phosphate | highly specific for 2-dehydro-3-deoxy-D-gluconate with up to 2000fold-higher catalytic efficiency compared to 2-dehydro-3-deoxy-6-phosphogluconate | Picrophilus torridus | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
4.2.1.39 | D-galactonate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
4.2.1.39 | gluconate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
4.2.1.39 | additional information | xylonate (10 mM) is not utilized | Picrophilus torridus | ? | - |
? | |
4.2.1.140 | D-galactonate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
4.2.1.140 | D-galactonate | the ratio of catalytic efficiency for gluconate and galactonate utilization is about 10 to 1 | Picrophilus torridus | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
4.2.1.140 | D-gluconate | - |
Picrophilus torridus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
4.2.1.140 | D-gluconate | the ratio of catalytic efficiency for gluconate and galactonate utilization is about 10 to 1 | Picrophilus torridus | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
4.2.1.140 | additional information | no activity with xylonate | Picrophilus torridus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.20 | homotetramer | 4 * 32000, SDS-PAGE | Picrophilus torridus |
4.1.2.51 | homotetramer | 4 * 32000, SDS-PAGE | Picrophilus torridus |
4.2.1.39 | homooctamer | 8 * 44000, SDS-PAGE | Picrophilus torridus |
4.2.1.140 | homooctamer | 8 * 44000, SDS-PAGE | Picrophilus torridus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.20 | 2-keto-3-deoxygluconate-specific aldolase | - |
Picrophilus torridus |
4.1.2.20 | KDG-specific aldolase | - |
Picrophilus torridus |
4.1.2.20 | KDGA | - |
Picrophilus torridus |
4.1.2.51 | KDG-specific aldolase | - |
Picrophilus torridus |
4.1.2.51 | KDGA | - |
Picrophilus torridus |
4.1.2.51 | Pto1279 | - |
Picrophilus torridus |
4.2.1.39 | GAD | - |
Picrophilus torridus |
4.2.1.39 | gluconate dehydratase | - |
Picrophilus torridus |
4.2.1.140 | Pto0485 | - |
Picrophilus torridus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.20 | 65 | - |
- |
Picrophilus torridus |
4.1.2.51 | 60 | - |
assay at | Picrophilus torridus |
4.1.2.51 | 65 | - |
- |
Picrophilus torridus |
4.2.1.39 | 70 | - |
half-life is about 15 min | Picrophilus torridus |
4.2.1.140 | 60 | - |
assay at | Picrophilus torridus |
4.2.1.140 | 70 | - |
- |
Picrophilus torridus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.20 | 70 | 90 | at 70°C, the enzyme does not lose activity upon incubation for 2 h. The half-lives of the enzyme at 80°C and 90°C are 20 min and 15 min, respectively | Picrophilus torridus |
4.1.2.51 | 70 | - |
2 h, stable | Picrophilus torridus |
4.1.2.51 | 80 | - |
half-life: 20 min | Picrophilus torridus |
4.1.2.51 | 90 | - |
half-life: 15 min | Picrophilus torridus |
4.2.1.39 | 60 | - |
no loss of activity upon incubation for 2 h | Picrophilus torridus |
4.2.1.140 | 60 | - |
2 h, stable | Picrophilus torridus |
4.2.1.140 | 70 | - |
half-life: 15 min | Picrophilus torridus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.20 | 0.34 | - |
2-dehydro-3-deoxy-6-phospho-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
4.1.2.20 | 26.7 | - |
2-dehydro-3-deoxy-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
4.1.2.51 | 0.34 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | pH 6.2, 60°C, enzyme purified from Picrophilus torridus | Picrophilus torridus | |
4.1.2.51 | 0.36 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | pH 6.2, 60°C, recombinant enzyme | Picrophilus torridus | |
4.1.2.51 | 24.2 | - |
2-dehydro-3-deoxy-D-gluconate | pH 6.2, 60°C, recombinant enzyme | Picrophilus torridus | |
4.1.2.51 | 26.7 | - |
2-dehydro-3-deoxy-D-gluconate | pH 6.2, 60°C, enzyme purified from Picrophilus torridus | Picrophilus torridus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.20 | 5.5 | - |
- |
Picrophilus torridus |
4.1.2.51 | 5.5 | - |
- |
Picrophilus torridus |
4.1.2.51 | 6.2 | - |
assay at | Picrophilus torridus |
4.2.1.39 | 6 | - |
- |
Picrophilus torridus |
4.2.1.140 | 6 | - |
- |
Picrophilus torridus |
4.2.1.140 | 6.2 | - |
assay at | Picrophilus torridus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.20 | 4.5 | 7.5 | - |
Picrophilus torridus |
4.1.2.51 | 4.5 | 7.5 | pH 4.5: 50% of maximal activity, pH 7.5: about 50% of maximal activity | Picrophilus torridus |
4.2.1.39 | 4.5 | 8.1 | 50% of activity at pH 8.1 and at pH 4.5 | Picrophilus torridus |
4.2.1.140 | 4.5 | 8.1 | pH 4.5: 50% of maximal activity, pH 8.1: about 50% of maximal activity | Picrophilus torridus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.2.20 | metabolism | Picrophilus torridus degrades glucose via a strictly nonphosphorylative Entner-Doudoroff pathway with a 2-keto-3-deoxygluconate-specific aldolase | Picrophilus torridus |
4.1.2.51 | physiological function | the enzyme is involved in the nonphosphorylative variant of the EntnerDoudoroff pathway | Picrophilus torridus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.20 | 0.04 | - |
2-dehydro-3-deoxy-6-phospho-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
4.1.2.20 | 89 | - |
2-dehydro-3-deoxy-D-gluconate | at 60°C in 50 mM sodium phosphate buffer (pH 6.2) | Picrophilus torridus | |
4.1.2.51 | 0.04 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | pH 6.2, 60°C, enzyme purified from Picrophilus torridus | Picrophilus torridus | |
4.1.2.51 | 0.14 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | pH 6.2, 60°C, recombinant enzyme | Picrophilus torridus | |
4.1.2.51 | 52 | - |
2-dehydro-3-deoxy-D-gluconate | pH 6.2, 60°C, recombinant enzyme | Picrophilus torridus | |
4.1.2.51 | 89 | - |
2-dehydro-3-deoxy-D-gluconate | pH 6.2, 60°C, enzyme purified from Picrophilus torridus | Picrophilus torridus |