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Literature summary extracted from

  • Marin, M.; Perez-Pantoja, D.; Donoso, R.; Wray, V.; Gonzalez, B.; Pieper, D.H.
    Modified 3-oxoadipate pathway for the biodegradation of methylaromatics in Pseudomonas reinekei MT1 (2010), J. Bacteriol., 192, 1543-1552.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.4 a 75-bp and a 125-bp fragment of the mmlJ gene are cloned into the vector pGEM-T Easy Pseudomonas reinekei

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.174 additional information generation of a mmlC knockout strain Pseudomonas reinekei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.4 10000
-
 determined by SDS-PAGE Pseudomonas reinekei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.174 4-methyl-3-oxoadipyl-CoA + CoA Pseudomonas reinekei
-
 methylsuccinyl-CoA + acetyl-CoA
-
 ?
2.8.3.6 succinyl-CoA + 3-oxoadipate Pseudomonas reinekei
-
 succinate + 3-oxoadipyl-CoA
-
 ?
5.3.3.4 (S)-5-oxo-2,5-dihydrofuran-2-acetate Pseudomonas reinekei
-
 5-oxo-4,5-dihydrofuran-2-acetate
-
 ?
5.3.3.4 3-methylmuconolactone Pseudomonas reinekei
-
 4-methyl-3-oxoadipate enol-lactone
-
 ?
5.3.3.4 muconolactone Pseudomonas reinekei
-
 3-oxoadipate enol-lactone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.174 Pseudomonas reinekei D2I940
-
-
2.8.3.6 Pseudomonas reinekei
-
-
-
3.1.1.24 Pseudomonas reinekei
-
-
-
5.3.3.4 Pseudomonas reinekei C5MR75
-
-
5.4.99.14 Pseudomonas reinekei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.4 MmlJ is partially purified by anion exchange chromatography using a MonoQ HR 5/5 column Pseudomonas reinekei
5.4.99.14 MonoQ column chromatography Pseudomonas reinekei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.174 4-methyl-3-oxoadipyl-CoA + CoA
-
 Pseudomonas reinekei methylsuccinyl-CoA + acetyl-CoA
-
 ?
2.8.3.6 succinyl-CoA + 3-oxoadipate
-
 Pseudomonas reinekei succinate + 3-oxoadipyl-CoA
-
 ?
3.1.1.24 3-methylmuconolactone + H2O
-
 Pseudomonas reinekei ?
-
 ?
3.1.1.24 4-methyl-3-oxoadipate enol-lactone + H2O
-
 Pseudomonas reinekei 4-methyl-3-oxoadipate + H+
-
 ?
5.3.3.4 (S)-5-oxo-2,5-dihydrofuran-2-acetate
-
 Pseudomonas reinekei 5-oxo-4,5-dihydrofuran-2-acetate
-
 ?
5.3.3.4 3-methylmuconolactone
-
 Pseudomonas reinekei 4-methyl-3-oxoadipate enol-lactone
-
 ?
5.3.3.4 muconolactone
-
 Pseudomonas reinekei 3-oxoadipate enol-lactone
-
 ?
5.4.99.14 3-methylmuconolactone
-
 Pseudomonas reinekei 4-methyl-3-oxoadipate enollactone
-
 ?
5.4.99.14 4-Carboxymethyl-4-methylbut-2-en-1,4-olide
-
 Pseudomonas reinekei 4-Carboxymethyl-3-methylbut-2-en-1,4-olide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.174 mmlC
-
 Pseudomonas reinekei
2.3.1.174 mmlC gene encoding for a 3-oxoadipyl-CoA thiolase Pseudomonas reinekei
2.8.3.6 3-oxoadipate:succinyl-coenzyme A transferase
-
 Pseudomonas reinekei
2.8.3.6 CatIJ
-
 Pseudomonas reinekei
2.8.3.6 PcaIJ
-
 Pseudomonas reinekei
3.1.1.24 4-methyl-3-oxoadipate enol-lactone hydrolase
-
 Pseudomonas reinekei
3.1.1.24 mmlL
-
 Pseudomonas reinekei
5.3.3.4 Methylmuconolactone isomerase
-
 Pseudomonas reinekei
5.3.3.4 MmlJ
-
 Pseudomonas reinekei
5.3.3.4 Muconolactone isomerase
-
 Pseudomonas reinekei
5.4.99.14 4-Methylmuconolactone methylisomerase
-
 Pseudomonas reinekei
5.4.99.14 methymuconolactone isomerase
-
 Pseudomonas reinekei
5.4.99.14 MmlJ
-
 Pseudomonas reinekei

General Information

EC Number General Information Comment Organism
5.3.3.4 metabolism the mml gene cluster consists of 10 genes encoding enzymes involved in the metabolism of 4-methylmuconolactone Pseudomonas reinekei