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Literature summary extracted from

  • Ambrosi, C.; Leoni, L.; Putignani, L.; Orsi, N.; Visca, P.
    Pseudobactin biogenesis, in the plant growth-promoting rhizobacterium Pseudomonas strain B10: Identification and functional analysis of the L-ornithine N5-oxygenase (psbA) gene (2000), J. Bacteriol., 182, 6233-6238.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.195 gene psbA, DNA and amino acid sequence determination and analysis, monocistronic gene Pseudomonas sp.

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.195 additional information a PsbA-defective mutant B10CA1 is fully complemented by addition of L-ornithine N5-oxide, the mutant strain shows impaired synthesis of fluorescent pigment and hydroxamate nitrogen in iron-poor medium, as well as defective biosynthesis of both forms of pseudobactin and salicylate-based siderophores, Fe3+ transfer is impaired, phenotype, overview Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.195 Iron the psbA expression is negatively controlled by iron Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.195 L-ornithine + NADPH + H+ + O2 Pseudomonas sp.
-
 N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
1.14.13.195 L-ornithine + NADPH + H+ + O2 Pseudomonas sp. B10
-
 N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.195 Pseudomonas sp. Q9F8X0 gene psbA
-
1.14.13.195 Pseudomonas sp. B10 Q9F8X0 gene psbA
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.13.195 flavoprotein
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.195 L-ornithine + NADPH + H+ + O2
-
 Pseudomonas sp. N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
1.14.13.195 L-ornithine + NADPH + H+ + O2 the enzyme contains the FATGY signature in the putative substrate binding pocket Pseudomonas sp. N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
1.14.13.195 L-ornithine + NADPH + H+ + O2
-
 Pseudomonas sp. B10 N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?
1.14.13.195 L-ornithine + NADPH + H+ + O2 the enzyme contains the FATGY signature in the putative substrate binding pocket Pseudomonas sp. B10 N5-hydroxy-L-ornithine + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.195 L-Orn N5-oxygenase
-
 Pseudomonas sp.
1.14.13.195 PsbA
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.195 FAD
-
 Pseudomonas sp.
1.14.13.195 NADPH
-
 Pseudomonas sp.

Expression

EC Number Organism Comment Expression
1.14.13.195 Pseudomonas sp. the psbA expression is negatively controlled by iron down