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Literature summary extracted from

  • Narita, Y.; Inouye, K.
    Kinetic analysis and mechanism on the inhibition of chlorogenic acid and its components against porcine pancreas alpha-amylase isozymes I and II (2009), J. Agric. Food Chem., 57, 9218-9225.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 caffeic acid mixed-type inhibition, almost all activities of isozymes PPA-I and PPA-II are lost in the presence of 4 mM Sus scrofa
3.2.1.1 chlorogenic acid 5-caffeoylquinic acid, mixed-type inhibition, complete inhibition of isozymes PPA-I is observed at 1.5 mM and that of isozyme PPA-II is at 2.0 mM Sus scrofa
3.2.1.1 quinic acid poor inhibitor, activities higher than 80% remain for both isozymes even in the presence of 15 mM quinic acid, while they decrease sharply when the quinic acid concentration increases from 15 to 35 mM. Complete inhibition of isozyme PPAI is given with 35.0 mM, and that of isozyme PPA-II is with 40 mM quinic acid Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 4.48
-
 4-nitrophenyl-alpha-D-maltoside isozyme PPA-I, at pH 6.9 and 30°C Sus scrofa
3.2.1.1 4.63
-
 4-nitrophenyl-alpha-D-maltoside isozyme PPA-II, at pH 6.9 and 30°C Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 55400
-
 SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 TSKgel BioAssist Q column chromatography, gel filtration Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 pancreas
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 4-nitrophenyl-alpha-D-maltoside + H2O
-
 Sus scrofa 4-nitrophenol + maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 PPA-I isozyme Sus scrofa
3.2.1.1 PPA-II isozyme Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 0.0494
-
 4-nitrophenyl-alpha-D-maltoside isozyme PPA-I, at pH 6.9 and 30°C Sus scrofa
3.2.1.1 0.0497
-
 4-nitrophenyl-alpha-D-maltoside isozyme PPA-II, at pH 6.9 and 30°C Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.1 0.21
-
 chlorogenic acid isozyme PPA-II, at pH 6.9 and 30°C Sus scrofa
3.2.1.1 0.23
-
 chlorogenic acid isozyme PPA-I, at pH 6.9 and 30°C Sus scrofa
3.2.1.1 1.12
-
 caffeic acid isozyme PPA-I, at pH 6.9 and 30°C Sus scrofa
3.2.1.1 1.64
-
 caffeic acid isozyme PPA-II, at pH 6.9 and 30°C Sus scrofa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.1 0.07
-
 isozyme PPA-II, at pH 6.9 and 30°C Sus scrofa chlorogenic acid
3.2.1.1 0.08
-
 isozyme PPA-I, at pH 6.9 and 30°C Sus scrofa chlorogenic acid
3.2.1.1 0.37
-
 isozyme PPA-II, at pH 6.9 and 30°C Sus scrofa caffeic acid
3.2.1.1 0.4
-
 isozyme PPA-I, at pH 6.9 and 30°C Sus scrofa caffeic acid
3.2.1.1 25.3
-
 isozyme PPA-II, at pH 6.9 and 30°C Sus scrofa quinic acid
3.2.1.1 26.5
-
 isozyme PPA-I, at pH 6.9 and 30°C Sus scrofa quinic acid