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Literature summary extracted from

  • Bielas, J.H.; Schmitt, M.W.; Icreverzi, A.; Ericson, N.G.; Loeb, L.A.
    Molecularly evolved thymidylate synthase inhibits 5-fluorodeoxyuridine toxicity in human hematopoietic cells (2009), Hum. Gene Ther., 20, 1703-1707.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.45 expressed in Escherichia coli strain K562 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.45 A17T/D116A/D254E the mutant shows decreased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme Homo sapiens
2.1.1.45 D254N the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme Homo sapiens
2.1.1.45 G52S the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme Homo sapiens
2.1.1.45 T51S the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme Homo sapiens
2.1.1.45 T51S/G52S the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme Homo sapiens
2.1.1.45 T51S/K82Q/K99E/N171S the mutant shows increased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme Homo sapiens
2.1.1.45 T53S/Y258F the mutant shows sensitivity towards 5-fluorodeoxyuridine similar to the wild type enzyme Homo sapiens
2.1.1.45 Y258F the mutant shows decreased sensitivity towards 5-fluorodeoxyuridine compared to the wild type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 5-fluorodeoxyuridine forms an inhibitory, ternary covalent complex with the enzyme Homo sapiens
2.1.1.45 5-fluorouracil
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 hematopoietic cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Homo sapiens dihydrofolate + dTMP
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.45 N5,N10-methylenetetrahydrofolate
-
 Homo sapiens