EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.1 | CMP | cytidine 5'-monophosphate, the product of sialyltransferase reactions, is not required by the trans-sialidase activity of the enzyme but enhances the trans-sialidase activity modestly as a non-essential activator. CMP can enhance the efficiency of the alpha2,6-sialidase activity of DELTA15Pd2,6ST in a dose-dependent manner | Photobacterium damselae | |
3.2.1.18 | CMP | cytidine 5'-monophosphate is not required by the trans-sialidase activity of the enzyme but enhances the trans-sialidase activity modestly as a non-essential activator | Photobacterium damselae |
EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.3.1 | synthesis | application of the alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST in the synthesis of sialosides | Photobacterium damselae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.3.1 | CDP | at a concentration of 1 mM inhibits both sialidase and trans-sialidase activities of DELTA15Pd2,6ST | Photobacterium damselae | |
2.4.3.1 | CTP | at a concentration of 1 mM inhibits both sialidase and trans-sialidase activities of DELTA15Pd2,6ST | Photobacterium damselae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | 53 | - |
Neu5Acalpha-4-nitrophenyl | alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST | Photobacterium damselae | |
3.2.1.18 | 53 | - |
4-nitrophenyl alpha-D-N-acetylneuraminic acid | - |
Photobacterium damselae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.3.1 | Photobacterium damselae | - |
- |
- |
3.2.1.18 | Photobacterium damselae | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.3.1 | additional information | DELTA15Pd2,6ST preferentially cleaves alpha2,6-linked sialic acid | Photobacterium damselae | ? | - |
? | |
2.4.3.1 | Neu5Acalpha-4-nitrophenyl + 4-methylumbelliferyl-beta-D-lactoside | alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST | Photobacterium damselae | ? | - |
? | |
3.2.1.18 | 4-nitrophenyl alpha-D-N-acetylneuraminic acid + H2O | - |
Photobacterium damselae | ? | - |
? | |
3.2.1.18 | alpha(2-6)-sialyllactose + H2O | DELTA15Pd2,6ST-catalyzed trans-sialidase reactions follow a ping-pong bi-bi reaction mechanism, DELTA15Pd2,6ST preferentially cleaves alpha2,6-linked sialic acid | Photobacterium damselae | sialic acid + lactose | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.3.1 | alpha2,6-sialyltransferase | - |
Photobacterium damselae |
3.2.1.18 | alpha2,6-sialidase | - |
Photobacterium damselae |
3.2.1.18 | alpha2,6-sialyltransferase | - |
Photobacterium damselae |
3.2.1.18 | alpha2,6-trans-sialidase | - |
Photobacterium damselae |
3.2.1.18 | DELTA15Pd2,6ST | the enzyme has additional alpha2,6-trans-sialidase and alpha2,6-sialidase activities | Photobacterium damselae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | 0.883 | - |
Neu5Acalpha-4-nitrophenyl | alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST | Photobacterium damselae | |
3.2.1.18 | 0.88 | - |
4-nitrophenyl alpha-D-N-acetylneuraminic acid | - |
Photobacterium damselae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.3.1 | 5.8 | - |
alpha2,6-sialidase and alpha2,6-trans-sialidase activities of DELTA15Pd2,6ST in MES buffer | Photobacterium damselae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.4.3.1 | physiological function | DELTA15Pd2,6ST-catalyzed trans-sialidase reaction follows a ping-pong bi-bi reaction mechanism. DELTA15Pd2,6ST also has alpha2,6-sialidase activity | Photobacterium damselae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.3.1 | 0.016 | - |
Neu5Acalpha-4-nitrophenyl | alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST | Photobacterium damselae |