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Literature summary extracted from

  • Yoshida, H.; Fuwa, T.J.; Arima, M.; Hamamoto, H.; Sasaki, N.; Ichimiya, T.; Osawa, K.; Ueda, R.; Nishihara, S.
    Identification of the Drosophila core 1 beta1,3-galactosyltransferase gene that synthesizes T antigen in the embryonic central nervous system and hemocytes (2008), Glycobiology, 18, 1094-1104.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.122 MnCl2 20 mM are included in assay medium Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine Drosophila melanogaster
-
 UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.122 Drosophila melanogaster Q7K237
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.122 central nervous system during embryogenesis, in late embryos at stage 16 Drosophila melanogaster
-
2.4.1.122 embryo
-
 Drosophila melanogaster
-
2.4.1.122 ML-DmBG3-c2 cell
-
 Drosophila melanogaster
-
2.4.1.122 S2 cell
-
 Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.122 0.000168
-
 the extracts of EY13370 third instar larvae have a galactosyltransferase activity of 44% of that of wild-type using para-nitrophenyl-N-acetyl-alpha-galactosaminide as the acceptor Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.122 UDP-galactose + glycoprotein N-acetyl-D-galactosamine
-
 Drosophila melanogaster UDP + glycoprotein D-galactosyl-(1->3)-N-acetyl-D-galactosamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.122 C1beta3GalT
-
 Drosophila melanogaster
2.4.1.122 core 1 beta1,3-galactosyltransferase
-
 Drosophila melanogaster
2.4.1.122 dC1beta3GalT1
-
 Drosophila melanogaster
2.4.1.122 T-synthase
-
 Drosophila melanogaster

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.122 25
-
 assay at Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.122 6.8
-
 assay at Drosophila melanogaster

General Information

EC Number General Information Comment Organism
2.4.1.122 malfunction mutant embryos show the loss of T antigens on the CNS and on a subset of hemocytes, 2 mutant lines, EY13370 and KG02976, have a P-element insertion in the CG9520 gene region, although the P-elements are located in the first intron of the gene in both lines, KG02976 homozygotes develop normally whereas some EY13370 homozygotes die during development and escaper homozygous EY13370 adult flies have abnormal legs, the levels of the enzyme transcripts in mutant third instar larvae homozygous for KG02976 or EY13370 are reduced to 60% and 15% of that of wild-type third instar larvae, terminal galactose of T antigen is defective in mutant embryos Drosophila melanogaster
2.4.1.122 physiological function enzyme is responsible for the synthesis and function of T antigen (mucin-type O-glycan) in vivo Drosophila melanogaster