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Literature summary extracted from

  • Chen, X.; Yauk, Y.; Nieuwenhuizen, N.; Matich, A.; Wang, M.; Perez, R.; Atkinson, R.; Beuning, L.
    Characterisation of an (S)-linalool synthase from kiwifruit (Actinidia arguta) that catalyses the first committed step in the production of floral lilac compounds (2010), Funct. Plant Biol., 37, 232-243.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.25 transient expression in Nicotiana benthamiana, His-tagged version expressed in Escherichia coli Actinidia arguta
4.2.3.25 transient expression in Nicotiana benthamiana, His-tagged version expressed in Escherichia coli Actinidia polygama

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.25 0.0021
-
 geranyl diphosphate 25 mM Mg2+ Actinidia arguta
4.2.3.25 0.0026
-
 geranyl diphosphate 25 mM Mg2+ Actinidia polygama
4.2.3.25 0.0266
-
 Mn2+ cofactor Mn2+, substrate 0.05 mM geranyl diphosphate Actinidia polygama
4.2.3.25 0.0282
-
 Mn2+ cofactor Mn2+, substrate 0.05 mM geranyl diphosphate Actinidia arguta
4.2.3.25 0.346
-
 Mg2+ cofactor Mg2+, substrate 0.05 mM geranyl diphosphate Actinidia arguta
4.2.3.25 0.891
-
 Mg2+ cofactor Mg2+, substrate 0.05 mM geranyl diphosphate Actinidia polygama

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.25 Mg2+ Mg2+ or Mn2+ required Actinidia arguta
4.2.3.25 Mg2+ Mg2+ or Mn2+ required Actinidia polygama
4.2.3.25 Mn2+ Mg2+ or Mn2+ required Actinidia arguta
4.2.3.25 Mn2+ Mg2+ or Mn2+ required Actinidia polygama

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.25 65000
-
 x * 65000, SDS-PAGE Actinidia arguta
4.2.3.25 65000
-
 x * 65000, SDS-PAGE Actinidia polygama

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.25 Actinidia arguta D4N3A0
-
-
4.2.3.25 Actinidia polygama D4N3A1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.25 Ni2+ affinity chromatography, gel filtration Actinidia arguta
4.2.3.25 Ni2+ affinity chromatography, gel filtration Actinidia polygama

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.25 flower
-
 Actinidia polygama
-
4.2.3.25 petal
-
 Actinidia arguta
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.25 0.013
-
 in the presence of Mg2+, 22% activity in the presence of Mn2+ Actinidia polygama
4.2.3.25 0.039
-
 in the presence of Mg2+, 50% activity in the presence of Mn2+ Actinidia arguta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.25 geranyl diphosphate + H2O
-
 Actinidia arguta (3S)-linalool + diphosphate
-
 ?
4.2.3.25 geranyl diphosphate + H2O
-
 Actinidia polygama (3S)-linalool + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.25 ? x * 65000, SDS-PAGE Actinidia arguta
4.2.3.25 ? x * 65000, SDS-PAGE Actinidia polygama

Synonyms

EC Number Synonyms Comment Organism
4.2.3.25 (S)-linalool synthase
-
 Actinidia arguta
4.2.3.25 (S)-linalool synthase
-
 Actinidia polygama

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.25 0.014
-
 geranyl diphosphate
-
 Actinidia polygama
4.2.3.25 0.044
-
 geranyl diphosphate
-
 Actinidia arguta

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.25 5.6
-
 geranyl diphosphate
-
 Actinidia polygama
4.2.3.25 21
-
 geranyl diphosphate
-
 Actinidia arguta