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Literature summary extracted from

  • Bregere, C.; Rebrin, I.; Gallaher, T.K.; Sohal, R.S.
    Effects of age and calorie restriction on tryptophan nitration, protein content, and activity of succinyl-CoA:3-ketoacid CoA transferase in rat kidney mitochondria (2009), Free Radic. Biol. Med., 48, 609-618.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.3.5 mitochondrion
-
Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.5 58000
-
2 * 58000, SDS-PAGE Rattus norvegicus
2.8.3.5 58000
-
2 * 58000, full-length protein, SDS-PAGE Rattus norvegicus
2.8.3.5 120000
-
gel filtration Rattus norvegicus
2.8.3.5 120000
-
MALDI-TOF mass spectrometry Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.3.5 succinyl-CoA + acetoacetate Rattus norvegicus
-
succinate + acetoacetyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.5 Rattus norvegicus
-
male Fischer 344 rats
-
2.8.3.5 Rattus norvegicus B2GV06
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.8.3.5 additional information the enzyme is a target for nitration, tryptophan 372, rather than a tyrosine residue, is the actual site of simultaneous additions of nitro and hydroxy groups, impact on enzyme activity, mass spectrometric analysis, overview Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.5 BioSep-SEC-S 3000 gel filtration Rattus norvegicus
2.8.3.5 native enzyme from mitochondria by chromatofocusing and gel filtration to over 75% purity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.3.5 kidney
-
Rattus norvegicus
-
2.8.3.5 kidney age-related decreases in SCOT protein amount and catalytic activity: between 4 and 24 months of age, the amounts of SCOT protein and catalytic activity decrease by 55 and 45%, respectively Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.3.5 additional information
-
-
Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.5 succinyl-CoA + acetoacetate
-
Rattus norvegicus succinate + acetoacetyl-CoA
-
?

Subunits

EC Number Subunits Comment Organism
2.8.3.5 dimer 2 * 58000, SDS-PAGE Rattus norvegicus
2.8.3.5 homodimer 2 * 58000, full-length protein, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.8.3.5 SCOT
-
Rattus norvegicus
2.8.3.5 succinyl-CoA:3-ketoacid CoA transferase
-
Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.3.5 30
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.3.5 8.5
-
assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.3.5 succinyl-CoA
-
Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.3.5 Rattus norvegicus isoelectric focusing
-
7

Expression

EC Number Organism Comment Expression
2.8.3.5 Rattus norvegicus between 4 and 24 months of age, the amount of the enzyme protein and catalytic activity decrease by 55% and 45%, respectively down

General Information

EC Number General Information Comment Organism
2.8.3.5 malfunction loss of succinyl-CoA:3-ketoacid CoA transferase protein in aged rats may attenuate the capacity of kidney mitochondria to utilize ketone bodies for energy production Rattus norvegicus
2.8.3.5 metabolism SCOT is a rate-limiting enzyme in the degradation of ketone bodies Rattus norvegicus
2.8.3.5 physiological function loss of SCOT protein in the aged rats may attenuate the capacity of kidney mitochondria to utilize ketone bodies for energy production Rattus norvegicus