EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.8.3.5 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.3.5 | 58000 | - |
2 * 58000, SDS-PAGE | Rattus norvegicus |
2.8.3.5 | 58000 | - |
2 * 58000, full-length protein, SDS-PAGE | Rattus norvegicus |
2.8.3.5 | 120000 | - |
gel filtration | Rattus norvegicus |
2.8.3.5 | 120000 | - |
MALDI-TOF mass spectrometry | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.5 | succinyl-CoA + acetoacetate | Rattus norvegicus | - |
succinate + acetoacetyl-CoA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.3.5 | Rattus norvegicus | - |
male Fischer 344 rats | - |
2.8.3.5 | Rattus norvegicus | B2GV06 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.8.3.5 | additional information | the enzyme is a target for nitration, tryptophan 372, rather than a tyrosine residue, is the actual site of simultaneous additions of nitro and hydroxy groups, impact on enzyme activity, mass spectrometric analysis, overview | Rattus norvegicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.3.5 | BioSep-SEC-S 3000 gel filtration | Rattus norvegicus |
2.8.3.5 | native enzyme from mitochondria by chromatofocusing and gel filtration to over 75% purity | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.8.3.5 | kidney | - |
Rattus norvegicus | - |
2.8.3.5 | kidney | age-related decreases in SCOT protein amount and catalytic activity: between 4 and 24 months of age, the amounts of SCOT protein and catalytic activity decrease by 55 and 45%, respectively | Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.3.5 | additional information | - |
- |
Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.3.5 | succinyl-CoA + acetoacetate | - |
Rattus norvegicus | succinate + acetoacetyl-CoA | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.3.5 | dimer | 2 * 58000, SDS-PAGE | Rattus norvegicus |
2.8.3.5 | homodimer | 2 * 58000, full-length protein, SDS-PAGE | Rattus norvegicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.8.3.5 | SCOT | - |
Rattus norvegicus |
2.8.3.5 | succinyl-CoA:3-ketoacid CoA transferase | - |
Rattus norvegicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.3.5 | 30 | - |
assay at | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.3.5 | 8.5 | - |
assay at | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.8.3.5 | succinyl-CoA | - |
Rattus norvegicus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.8.3.5 | Rattus norvegicus | isoelectric focusing | - |
7 |
EC Number | Organism | Comment | Expression |
---|---|---|---|
2.8.3.5 | Rattus norvegicus | between 4 and 24 months of age, the amount of the enzyme protein and catalytic activity decrease by 55% and 45%, respectively | down |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.8.3.5 | malfunction | loss of succinyl-CoA:3-ketoacid CoA transferase protein in aged rats may attenuate the capacity of kidney mitochondria to utilize ketone bodies for energy production | Rattus norvegicus |
2.8.3.5 | metabolism | SCOT is a rate-limiting enzyme in the degradation of ketone bodies | Rattus norvegicus |
2.8.3.5 | physiological function | loss of SCOT protein in the aged rats may attenuate the capacity of kidney mitochondria to utilize ketone bodies for energy production | Rattus norvegicus |