Any feedback?
Literature summary extracted from
- An enzyme possessing both glutathione-dependent formaldehyde dehydrogenase and S-nitrosoglutathione reductase from Antrodia camphorata (2009), Food Chem., 112, 795-802.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.284 | subcloned into NdeI and NotI sites of pET-20b(+) vector and overexpressed in Escherichia coli BL21(DE)pLysS | Taiwanofungus camphoratus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.284 | 0.28 | - |
S-nitrosoglutathione | pH 8.0, 25°C | Taiwanofungus camphoratus |  |
| 1.1.1.284 | 1.2 | - |
S-(hydroxymethyl)glutathione | pH 8.0, 25°C | Taiwanofungus camphoratus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.284 | Taiwanofungus camphoratus | D2CFJ2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.284 | by Ni-NTA affinity chromatography | Taiwanofungus camphoratus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.284 | S-(hydroxymethyl)glutathione + NAD+ | - |
Taiwanofungus camphoratus | S-formylglutathione + NADH + H+ | - |
? | |
| 1.1.1.284 | S-nitrosoglutathione + NADH + H+ | - |
Taiwanofungus camphoratus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.284 | GFD | - |
Taiwanofungus camphoratus |
| 1.1.1.284 | Glutathione-dependent formaldehyde dehydrogenase | - |
Taiwanofungus camphoratus |
| 1.1.1.284 | GSH-FDH | - |
Taiwanofungus camphoratus |
| 1.1.1.284 | GSNOR | - |
Taiwanofungus camphoratus |
| 1.1.1.284 | S-nitrosoglutathione reductase | - |
Taiwanofungus camphoratus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.284 | NADH | - |
Taiwanofungus camphoratus | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.284 | physiological function | the enzyme GFD is bifunctional. In addition to the glutathione-dependent formaldehyde dehydrogenase activity, it also functions as an effective S-nitrosoglutathione reductase presumably to safeguard against nitrosative stress | Taiwanofungus camphoratus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
