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Literature summary extracted from

  • Li, A.; Qu, Y.; Zhou, J.; Ma, F.
    Enzyme-substrate interaction and characterization of a 2,3-dihydroxybiphenyl 1,2-dioxygenase from Dyella ginsengisoli LA-4 (2009), FEMS Microbiol. Lett., 292, 231-239.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 subcloned into the pET28(a) vector, yielding pET-bphC and overexpressed in Escherichia coli BL21 (DE3) Dyella ginsengisoli

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.39 Cu2+
-
 Dyella ginsengisoli
1.13.11.39 H2O2
-
 Dyella ginsengisoli
1.13.11.39 KI 600 mM reduces specific activity by 35% Dyella ginsengisoli
1.13.11.39 L-ascorbic acid completely inhibits at concentrations over 25 mM Dyella ginsengisoli
1.13.11.39 SDS 1.25 mM inhibits nearly 60% Dyella ginsengisoli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.0189
-
 2,3-Dihydroxybiphenyl pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 0.0496
-
 4-Chlorocatechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 0.0688
-
 3-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 0.281
-
 catechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 0.3597
-
 4-methylcatechol pH 8.0, 20°C Dyella ginsengisoli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Ca2+
-
 Dyella ginsengisoli
1.13.11.39 Fe2+ is an iron (II)-dependent type I extradiol dioxygenase Dyella ginsengisoli
1.13.11.39 Mg2+
-
 Dyella ginsengisoli
1.13.11.39 additional information Ba2+ has no effect Dyella ginsengisoli

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Dyella ginsengisoli A9QT35 LA-4
-
1.13.11.39 Dyella ginsengisoli LA-4 A9QT35 LA-4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.39 by gel filtration, more than 95% pure Dyella ginsengisoli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 118.3
-
 with 2,3-dihydroxybiphenyl as substrate Dyella ginsengisoli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 2,3-dihydroxybiphenyl + O2 best substrate Dyella ginsengisoli 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2,3-dihydroxybiphenyl + O2 best substrate Dyella ginsengisoli LA-4 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Dyella ginsengisoli 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Dyella ginsengisoli LA-4 2-hydroxy-6-oxohepta-2,4-dienoate
-
 ?
1.13.11.39 4-chlorocatechol + O2
-
 Dyella ginsengisoli 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.39 4-chlorocatechol + O2
-
 Dyella ginsengisoli LA-4 4-chloro-2-hydroxy-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Dyella ginsengisoli 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Dyella ginsengisoli LA-4 2-hydroxy-4-methyl-6-oxohexa-2,4-dienoate
-
 ?
1.13.11.39 catechol + O2
-
 Dyella ginsengisoli ?
-
 ?
1.13.11.39 catechol + O2
-
 Dyella ginsengisoli LA-4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 2,3-dihydroxybiphenyl 1,2-dioxygenase
-
 Dyella ginsengisoli
1.13.11.39 BphC
-
 Dyella ginsengisoli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.39 40
-
-
 Dyella ginsengisoli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.39 20 70 20°C: about 75% of maximal activity, 70°C: about 85% of maximal activity Dyella ginsengisoli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.39 0.7
-
 4-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 1
-
 4-Chlorocatechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 6.7
-
 catechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 16
-
 3-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 84.1
-
 2,3-Dihydroxybiphenyl pH 8.0, 20°C Dyella ginsengisoli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.39 8
-
-
 Dyella ginsengisoli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.39 7 9 pH 7.0: about 50% of maximal activity, pH 9.0: about 45% of maximal activity Dyella ginsengisoli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.39 2
-
 4-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 20
-
 4-Chlorocatechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 24
-
 catechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 233
-
 3-methylcatechol pH 8.0, 20°C Dyella ginsengisoli
1.13.11.39 4450
-
 2,3-Dihydroxybiphenyl pH 8.0, 20°C Dyella ginsengisoli